人肺癌组织中富含酪氨酸61和结缔组织生长因子的表达

目的:研究人肺癌组织中富含酪氨酸61(CYR61)和结缔组织生长因子(CTGF)的基因表达及其与临床参数的关系.方法:采用实时定量PCR及免疫组织化学技术对60例肺癌患者的肺癌组织和癌旁正常肺组织CYR61和CTGF基因mRNA表达水平和蛋白表达进行测定,并分析二者的表达与临床参数的关系.结果:肺癌组织中CYR61和CTGF mRNA表达水平和蛋白阳性表达率均低于癌旁正常组织(P均＜0.05).肺癌组织中CYR61和CTGF mRNA的表达水平与患者的病理类型、病理分级、淋巴结转移、吸烟及家族史等临床参数有关(P＜0.05);肺癌组织中CYR61和CTGF mRNA表达水平呈正相关(r=0.604;P=0.000).多元回归分析:病理类型和年龄对CYR61表达有重要影响,肿瘤的位置、CYR61表达和性别对肺癌组织中的CTGF表达有重要影响.结论:CYR61和CTGF单种基因或两者相互作用对肺癌的发展起重要作用,其表达的蛋白产物可作为临床干预治疗的重要靶蛋白.

Expression of CYR61 and CTGF in human lung cancer tissue

Aim: To investigate CYR61 and CTGF expression levels and their relation to clinical and pathological features in lung cancer.Methods: The mRNA and protein expression levels of CYR61 and CTGF were determined in 60 cases of lung cancer tissue and their matched lung tissue using real-time PCR and immunohistochemistry staining. Results: The mRNA and protein expression levels in lung cancer tissue were both lower than those in lung tissue (P<0.05). The mRNA expression of CYR61 and CTGF was related to histological types, tumor grade, lymph node metastasis, smoking, and family history (P<0.05). The mRNA expression of CYR61 was positively correlated with that of CTGF(r=0.604;P=0.000). Multiple linear regression analysis revealed that tumor type and age were important for predicting CYR61 expression in lung cancer, while tumor position, expression of CYR61, and gender were influencing factors for CTGF expression.Conclusion: CYR61 and CTGF may play a role in the progression of lung cancer by either single gene itself or co-interactions of the two genes, and their proteins might serve as valuable targets for therapeutic intervention.