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红大戟的组织培养及植株再生
引用本文:凌征柱,覃文流,余丽莹,蓝祖栽,吴庆华.红大戟的组织培养及植株再生[J].中草药,2005,36(10):1555-1557.
作者姓名:凌征柱  覃文流  余丽莹  蓝祖栽  吴庆华
作者单位:1. 广西药用植物园,广西,南宁,530023
2. 广西大学,广西,南宁,530005
基金项目:广西壮族自治区科技厅科技攻关项目(0322024-3A)
摘    要:目的研究红大戟快速繁殖技术,为人工栽培提供种源。方法以野生红大戟的茎尖作外植体,通过不同的培养基进行诱导培养获得丛生芽,进而生根获得再生植株。结果M S BA 2.0 m g/L NAA 0.1 m g/L适合红大戟的丛生芽继代增殖。1/2M S NAA 1.0 m g/L适宜诱导生根获得健全再生植株。移栽成活率70%。结论本研究得出的方法可为人工栽培红大戟提供种源。

关 键 词:红大戟  组织培养  试管苗
文章编号:0253-2670(2005)10-1555-03
收稿时间:2004-12-27
修稿时间:2004-12-27

Tissue culture and plantlet regeneration of Knoxia valerianoides
LING Zheng-zhu,QIN Wen-liu,YU Li-ying,LAN Zu-zai and WU Qing-hua.Tissue culture and plantlet regeneration of Knoxia valerianoides[J].Chinese Traditional and Herbal Drugs,2005,36(10):1555-1557.
Authors:LING Zheng-zhu  QIN Wen-liu  YU Li-ying  LAN Zu-zai and WU Qing-hua
Institution:1. Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China;2. Guangxi University, Nanning 530005, China
Abstract:Objective The tissue culture of the stem apex of Knoxia valerianoides was done to research the optimum culture medium for test-tube seedlings for large-scale planting.Methods The stem apex of wild K.valerianoides was used as the explants and cultivated in different culture media separately to induce fascicled-bud,root,and plantlet regeneration.Results The cultural medium MS BA 2.0 mg/L NAA 0.1 mg/L was suitable for the sub-multiplication of fascicled bud and the medium 1/2MS NAA 1.0 mg/L for root inducement and the plantlet regeneration.The transplantation survival rate of the plantlet regeneration was 70%.Conclusion The tissue culture of K.valerianoides could be used to produce test-tube seedlings for large-scale planting.
Keywords:Knoxia valerianoides Thorel et Pitard  tissue culture  test-tube seedling  
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