不同月龄大鼠勃起功能状况的实验研究

目的:探讨不同月龄组大鼠勃起功能的变化规律,为建立增龄性大鼠ED模型提供动物实验依据。方法:80只雄性Wistar大鼠,分为3、6、12和18月龄组,分别灌胃枸橼酸西地那非进行阴茎勃起功能实验;20只3月龄雌性Wistar大鼠,随机分为4组,建立发情雌鼠模型,观察不同月龄雄鼠的勃起状况与勃起次数。结果:3、6、12和18月龄组Wistar大鼠勃起率及勃起次数分别为85%、75%、40%、30%及(2.27±0.80)、(2.00±0.61)、(1.40±0.51)、(1.29±0.49)次,不同月龄勃起率与勃起次数比较,差异有统计学意义(P<0.05),且随着月龄的增加,各组大鼠勃起功能有降低的趋势。分别将6、12、18月龄组的勃起率及勃起次数与3月龄组大鼠做两两比较,发现6月龄组与12月龄组较3月龄组的差异无统计学意义(P>0.05);18月龄组较3月龄组有明显降低,且差异有显著性(P<0.05)。结论:衰老是大鼠发生ED的主要危险因素,18月龄大鼠已具备建立增龄性大鼠ED模型的条件。

Erectile function of male rats in different age groups: An experimental study

Objective: To explore the relationship between aging and erectile function changes in rats in order to establish a rat model of aging-related erectile dysfunction(ED).Methods: Eighty male Wistar rats were equally divided into four age groups(3-,6-,12-and 18-month) and treated with intragastric administration of sildenafil ciltrate(Sn) for penile erection tests.Twenty 3-month-old female Wistar rats were randomized to four groups as oestrous rat models.We recorded the rate and frequency of penile erections of the male rats in different age groups.Results: The rates of penile erection were 85%,75%,40% and 30% and erectile frequencies were 2.27 ± 0.80,2.00 ± 0.61,1.40 ± 0.51 and 1.29 ± 0.49 in the 3-,6-,12-and 18-month rats,respectively,with statistically significant differences among different age groups(P<0.01).And their erectile function exhibited a tendency to decrease with the increase of age.Besides,comparison of the 3-month with the 6-,12-and 18-month groups showed significantly reduced erectile function in the 18-month rats(P<0.05) but no remarkable difference between the 3-month and the 6-and 12-month groups(P>0.05).Conclusion: Aging is one of the main risk factors of rat erectile dysfunction,and 18-month-old male rats are qualified for the establishment of the rat model of aging-related erectile dysfunction.