乙醛脱氢酶1A1过表达对胃癌细胞生物学行为的影响

目的 探讨乙醛脱氢酶1A1（ALDH1A1）过表达对胃癌细胞增殖、克隆形成和侵袭能力的影响。方法 成功构建过表达ALDH1A1的pEGFP-N1-ALDH1A1真核载体并转染至人胃癌细胞株MKN-28（实验组），同时设空载体组（转染空载体pEGFP-N1的MKN-28细胞）和空白对照组（未行任何干预措施的MKN-28细胞），分别于转染96 h后采用四甲基偶氮唑盐（MTT）法、克隆形成实验及Transwell小室侵袭实验检测ALDH1A1过表达对MKN-28细胞增殖、克隆形成和侵袭能力的影响。结果 实验组转染96 h后的增殖抑制率为（19.26±2.01）％，均低于空载体组和空白对照组，而克隆形成率和穿膜细胞数分别为（25.44±1.74）％和（219.78±12.93）个，均高于空载体组和对照组，差异有统计学意义（P＜0.05）。空白对照组和空载体组以上指标的差异均无统计学意义（P＞0.05）。结论 在MKN-28细胞中过表达ALDH1A1，可增强肿瘤细胞的增殖、克隆形成和侵袭能力，提示ALDH1A1可能参与了胃癌的发生、发展。

Influence of ALDH1A1 overexpression on biological behavior of gastric cancer cells

Objective To evaluate the effect of overexpression of aldehyde dehydrogenases 1A1（ALDH1A1）on the proliferation，clone formation and invasion of human gastric cancer cell.Methods The eukaryotic expression vector，pEGFP-N1-ALDH1A1，was transfected into gastric cancer cell line MKN-28（experimental group）. The empty vector group（MKN-28 cells transfected with empty vector pEGFP N1）and blank control group（MKN-28 cells without any treatment）were also prepared. The methyl thiazolyl tetrazolium（MTT），clone formation assay and Transwell chamber invasion assay were used to evaluate the cell proliferation，clone formation and invasion ability at 96 h after transfection，respectively. Results The proliferation inhibition rate of experimental group was（19.26±2.01）％，lower than that in empty vector group and blank control group. While the clone formation rate and number of cells penetrating membrane were（25.44±1.74）％ and 219.78±12.93，higher than those in the empty vector group and blank control group（P＜0.05）. No significant difference was observed on the above items between blank control group and empty vector group（P＞0.05）. Conclusion Overexpression of ALDH1A1 can increase the proliferation，colony formation and invasion ability of human gastric cancer cell MKN-28.ALDH1A1 may be involved in the occurence and development of gastric cancer.