miRNA-29s在非小细胞肺癌组织中的表达及临床意义

目的 探讨非小细胞肺癌（NSCLC）组织中微小RNA-29s（miR-29s）的表达水平及临床意义。方法 收集2012年1月至2015年12月收治的94例手术切除的NSCLC组织及71例癌旁正常组织，采用实时荧光定量PCR（QPCR）法检测上述组织中miR-29a、miR-29b和mi-29c的表达，比较NSCLC组织及癌旁正常组织中miR-29s的表达水平，Pearson相关分析NSCLC组织中miR-29a、miR-29b和miR-29c表达的相关性，分析NSCLC组织中miR-29s的表达与NSCLC临床病理特征（性别、年龄、TNM分期、组织学类型、分化程度、淋巴结转移及CEA水平）的关系，根据随访资料分析不同miR-29s表达水平与预后的关系。结果 QPCR结果显示，NSCLC组织中miR-29a、miR-29b和miR-29c的表达水平依次为0.413±0.025、0.609±0.039和0.161±0.013，均低于癌旁正常组织，差异有统计学意义（P＜0.05）；NSCLC组织中miR-29a与miR-29b呈正相关（r=0.637，P=0.000），miR-29a与miR-29c呈正相关（r=0.775，P=0.000），miR-29b与miR-29c呈正相关（r=0.586，P=0.000）；NSCLC组织中miR-29s表达均与TNM分期有关，miR-29a与肿瘤大小及分化程度有关，miR-29b与分化程度有关，miR-29c与肿瘤大小有关。miR-29a（≥0.354）、miR-29b（≥0.586）和miR-29c（≥0.118）高水平者的中位总生存时间依次为16.4、16.4和16.7个月，均高于低水平者的11.5、9.8和10.5个月，差异有统计学意义（P＜0.05）。结论 miR-29s在NSCLC组织中表达降低，且均与TNM分期及预后有关，可能与NSCLC发生、发展有关，对NSCLC诊断及病情评估有一定价值。

Expression of miRNA-29s in non-small cell lung cancer and its clinical significance

Objective To investigate the expression of miRNA-29s (miR-29s) and its clinical significance in non-small cell lung cancer (NSCLC).Methods From January 2012 to December 2015,94 cases of surgical resected NSCLC tissues and 71 cases of adjacent normal tissues were collected.The levels of miR-29a,miR-29b and miR-29c in the above tissues were detected by fluorescence real-time quantitative PCR (QPCR) method.The miR-29s levels in NSCLC tissues and adjacent normal tissues were compared.The correlation among miR-29a,miR-29b and miR-29c were analyzed by Pearson correlation analysis.The relationship between miR-29s level and NSCLC clinicopathological parameters (gender,age,TNM stage,histological type,differentiation degree,lymph node metastasis and CEA level) were also analyzed.The relationship between different miR-29s levels and prognosis were compared according to follow-up data.Results The QPCR results showed that the levels of miR-29a,miR-29b and miR-29c in NSCLC tissues were 0.413±0.025,0.609±0.039 and 0.161±0.013,all lower than those in adjacent normal tissues with statistical significance (P＜ 0.05).MiR-29a level of NSCLC was positively correlated with miR-29b (r =0.637,P =0.000),and miR-29a levels were positively correlated with miR-29c (r=0.775,P=0.000),and miR-29c levels were positively correlated with miR-29b (r=0.586,P=0.000).In NSCLC tissues,miR-29s levels were related to TNM stage.Additionally,miR-29a was related to tumor size and differentiation degree,miR-29b was related to the degree of differentiation,and miR-29c was related to tumor size.The median overall survival of NSCLC with higher levels of miR-29a(≥0.354),miR-29b(≥0.586) and miR-29c(≥0.118) were 16.4,16.4 and 16.7 months,better than 11.5,9.8 and 10.5 months of NSCLC with lower levels of miR-29a,miR-29b and miR-29c (P＜0.05).Conclusion Decreased expression of miR-29s were observed in NSCLC tissue,and they were related to TNM staging and prognosis.They may be related to the occurrence and development of NSCLC,which have a certain value in the diagnosis and assessment of NSCLC.