大鼠杏仁中央核神经紧张肽和强啡肽神经元的年龄变化及牛膝的干预作用-免疫组织化学及图像定量分析研究

采用免疫组织化学ABC法结合图像定量分析观察杏仁中央核内神经紧张肽和强啡肽标记神经元的年龄变化及牛膝的抗衰老作用.结果:神经紧张肽神经元随月龄增加呈现细胞数减少、细胞面积增加和灰度值增大(P<0.05),22月龄大鼠发现有形态改变.强啡肽神经元左右两侧不同步变化,左侧在9月龄呈现细胞数减少,细胞面积增加、灰度值增大(P<0.05),在22月龄还可见形态改变;右侧在22月才出现细胞数减少、细胞面积增加、灰度值增大(P<0.05),未见明显的形态改变.牛膝喂药组与同月龄大鼠相比,神经紧张肽和强啡肽神经元的细胞数减少、细胞面积增加和灰度值的增大均降低(P<0.05),未见明显的形态改变,证实牛膝有延缓衰老之功效.

AGING CHANGES OF NEUROTENSIN-LIKE AND DYNORPHIN-LIKE NEURONS IN THE CENTRAL NUCLEUS OF AMYGDALA OF THE RAT ANTI-AGING EFFECT OF RADIX ACHYRANTHIS BIDENTATAE-IMMUNOHISTOCHEMICAL AND IMAGE QUANTITATIVE ANALYTICAL STUDY

By using ABC immunohistochemical method and image quantitative analysis, the aging changes of neurotensin-like (NT) and dynorphin-like (DYN) neurons of the central nucleus of amygdala and the anti- = aging effect of radix achyranthis bidentatae (RAB) were observed. The results were as fellows: The number of NT neurons decreased with age, but the average area and grey value of NT neurons increased markedly (P<0. 05). Morphological changes were found in the rats of 22 months. Changes of the DYN neurons were not synchronized bilaterally. On the left side, in the rats of 9 to 22 months, the number of DYN neurons decreased while the average area and grey value increased markedly (P<0. 05). On the right side, there were not such changes until 22 months. By contrast, there weresignificant increase in number and decrease in the average area and grey value of both NT and DYN neurons in groups fed with RAB, but there were not significantly morphological changes. The result suggests that RAB has the anti-aging effect.