补骨脂对大鼠胆汁分泌和豚鼠胆囊平滑肌收缩力的影响

目的研究补骨脂生药粉对大鼠胆汁分泌和豚鼠胆囊平滑肌收缩力的影响。方法大鼠连续灌胃给予补骨脂生药粉（2．1g·kg-1）30天。同时设对照组。末次给药后用水合氯醛麻醉动物，进行胆总管插管。收集胆汁，比较补骨脂组与对照组在同时间点胆汁量和总胆汁量的差异。豚鼠禁食24小时后击头致毙．迅速取出胆囊．制成平滑肌条，观察不同浓度补骨脂液对平滑肌收缩力的影响。结果补骨脂2．1g·kg-1组大鼠胆汁分泌量与对照组之间无显著差异。补骨脂4．8g·L-1、2．4g·L-1、1．2g·L-1能显著增高豚鼠离体胆囊平滑肌条张力，与剂量呈现一定的正相关性。结论补骨脂对大鼠胆汁分泌没有显著影响，但是可以促进豚鼠胆囊平滑肌的收缩。

Effect of Fructus Psoraleae on rats' bile secretion and contractile activity f guinea-pigs' gallbladder smooth muscle

OBJECTIVE To investigate the effects of Fructus Psoraleae crude powder on rats＇ bile secretion and contractile activity of guinea-pigs＇ gallbladder smooth muscle. METHODS Rats were intragastric administra- tion for 30 days with Fructus Psoraleae crude powder （2. 1 g· kg-1） and water （control）. Animals were anesthesia by chloral hydrate after the end of last administration. The output of bile secretion at the same period, the total quantity of bile secretion were compared in Fructus Psoraleae group and control group, by collecting the bile fluid from the common choledochal ductus. The guinea pigs were killed after fasting for 24 hours. The gallbladders were extracted rapidly and the muscle strips were prepared to investigate the effect on contraction of gallbladder smooth muscle of different concentration of Fructus Psoraleae. RESULTS Compared with the control group, there were no significant changes on the rats＇ bile secretion. The tension of gallbladder smooth muscle increased significantly in the drug groups with Fructus Psoraleae of 4.8g·L-1, 2.4g·L-1, 1.2g·L-1 . The effect was dose depen- der~.CONCLUSION The Fructus Psoraleae increased contractile activity of guinea pigs＇ gallbladder smooth muscle, while there was no significant changes on the bile secretion in rats.