Wnt3蛋白在非小细胞肺癌中的表达及临床意义

目的：研究Wnt3蛋白在非小细胞肺癌（non-small cell lung cancer，NSCLC）中的表达水平及临床意义。方法：采用免疫组织化学方法对64例NSCLC患者术后标本进行Wnt3蛋白表达水平的检测。结合阳性细胞染色强弱及百分比计算Wnt3免疫组化染色评分，分析Wnt3表达与患者临床病理特征之间的关系，并探讨Wnt3蛋白在NSCLC患者中表达的意义。结果：在NSCLC组织中，Wnt3的免疫组化染色定位于胞浆中，其阳性表达率为64.1%（41/64），显著高于癌旁组织阳性表达率40.0%（12/30）（P=0.028）。Wnt3蛋白表达强度与NSCLC的分化程度（P=0.028）、淋巴结转移（P=0.008）和病理分期（P=0.000）有显著相关性，而与NSCLC的年龄、性别、吸烟状态、组织类型及侵润深度（T分期）无关（P均>0.05）。结论：Wnt3蛋白在NSCLC中高表达，可能与NSCLC的发生发展相关。

The expression and clinical significance of Wnt3 in non-small cell lung cancer

Objective: To study the expression and clinical significance of Wnt3 in non-small cell lung cancer (NSCLC). Methods: Immunohistochemistry was used to detect the expression of Wnt3 protein in 64 specimens of NSCLC patients. The expression of Wnt3 immunohistochemical staining was calculated with the percentage and staining intensity index of positive cells staining. Chi-square test was used to analyze the relationship between Wnt3 expression and clinicopathological features of the patients, and the correlation of Wnt3 expression in NSCLC patients was explored. Results: In NSCLC tissues, the immunohistochemical staining of Wnt3 was localized in the cytoplasm, and the positive expression rate of Wnt3 was 64.1% (41/64), significantly higher than that of paracancerous tissues,which was 40.0% (12/30) (P=0.028). The expression of Wnt3 protein was significantly correlated with the degree of NSCLC differentiation (P=0.028), lymph node metastasis (P=0.008) and pathological stage (P=0.000).However,there was no significant association with age, gender, smoking status, tissue type, and NSCLC infiltration depth. Conclusion: Wnt3 protein is highly expressed in NSCLC and may be related to the development of NSCLC.