蛇床子素对过氧化氢诱导下人表皮黑素细胞氧化损伤的影响

目的探讨中药单体蛇床子素对过氧化氢诱导下人表皮黑素细胞氧化损伤的影响。方法体外培养人表皮黑素细胞,并用L-DOPA鉴定。用一定浓度蛇床子素预处理黑素细胞,并用过氧化氢构建黑素细胞氧化损伤模型,采用MTS法检测黑素细胞活力,倒置显微镜下观察黑素细胞树突变化,并记录树突变化率。分别用WST-1法和TBA法,测定细胞内SOD及MDA含量。结果与对照组相比,过氧化氢组黑素细胞活力及细胞内SOD活性显著下降(P <0. 05),经蛇床子素预处理的黑素细胞活力及SOD活性虽低于对照组,但显著高于过氧化氢组(P <0. 05)。与对照组相比,过氧化氢组黑素细胞树突变化率及细胞内MDA含量明显升高(P <0. 05),经蛇床子素预处理的黑素细胞树突变化率及细胞内MDA含量虽比对照组升高,但显著低于过氧化氢组(P <0. 05)。结论蛇床子素可抑制过氧化氢对黑素细胞的氧化损伤作用,对黑素细胞活力及树突的变化具有保护作用,并可升高细胞内SOD水平,降低MDA水平。

Effect of Osthole on Oxidative Damage of Human Epidermal Melanocytes Induced by Hydrogen Peroxide

Objective To investigate the protective effect of the osthole on oxidative damage induced by hydrogen peroxide(H2O2)in human epidermal melanocytes(MCs).Methods MCs were cultured and identified by L-DOPA staining.The cells were treated with osthole,and the model of oxidative damage induced by H2O2 was established.The viability of MCs was determined by MTS assay.The dendrites in cells were observed by microscopy,and the rate of change was calculated.Superoxide dismutase(SOD)activity and malondialdehyde(MDA)levels in MCs were determined using the WST-1 and TBA methods,respectively.Results In comparison with the control group,the viability of MCs and SOD activity were significantly increased in the H2O2 group(P<0.05)and significantly increased in the osthole group(P<0.05).In comparison with the control group,the rate of change of cell dendrites and levels of MDA were significantly increased in the H2O2 group(P<0.05)and significantly decreased in the osthole group(P<0.05).Conclusions Osthole can reduce oxidative damage induced by H2O2 in MCs,and protect viability of cell and dendrites,increase SOD activity and decrease the level of MDA.