金雀异黄素下调Gli1表达抑制MHCC97H细胞系肝癌干细胞样细胞侵袭

目的:研究金雀异黄素能否和如何抑制肝细胞癌MHCC97H细胞系肝癌干细胞样细胞侵袭。方法:干细胞条件培养基悬浮培养MHCC97H细胞系球形成细胞,作为肝癌干细胞样细胞。不同浓度金雀异黄素处理后,Transwell小室侵袭试验检测体外细胞侵袭能力;Western blot分析Gli1和Snail1蛋白表达。结果:干细胞条件培养基悬浮培养MHCC97H细胞能形成肿瘤球。金雀异黄素(5、10和20μM)作用第3代球形成细胞即肝癌干细胞样细胞72 h降低肿瘤球形成率和细胞侵袭率;呈浓度依赖性。金雀异黄素(5、10和20μM)孵育肝癌干细胞样细胞24 h下调Gli1和Snail1蛋白表达。结论:金雀异黄素可能通过调控Gli1抑制Snail1蛋白表达抑制肝癌干细胞样细胞侵袭。

Genistein Inhibits the Invasion of Hepatic Cancer Stem-Like Cells from MHCC97H Cell Line Via Down-Regulating Gli1

Objective To investigate whether and how genistein suppresses the invasion of hepatic cancer stem-like cells( HCSLCs) from MHCC97 H cell line. Methods Sphere-forming cells( SFCs) derived from MHCC97 H cell line which is also called HCSLCs were obtained though suspended culture with stem cell-conditioned medium. After treatment with various concentrations of genistein,the capability of invasion was tested using Transwell invasion assay. And the protein expression of Gli1 and Snail1 was analyzed by western blot. Results MHCC97 H cells can form tumor spheres though suspended culture with stem cell-conditioned medium. After treatment with genistein( 5,10 and 20 μM) for 72 h,the rate of sphere-forming and invasion was decreased in SFCs namely HCSLCs,in a concentration-dependent manner. And the protein expression of Gli1 and Snail1 was down-regulated in HCSLCs. Conclusion Effects of genistein on inhibiting invasion of HCSLCs is likely involved in the reduced protein expression of Snail1 by regulating Gli1.