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| 实时荧光定量PCR技术进展 | |
| 引用本文： | 洪云,李津,汪和睦,赵铠. 实时荧光定量PCR技术进展[J]. 国际流行病学传染病学杂志, 2006, 33(3): 161-163,166 |
| 作者姓名： | 洪云 李津 汪和睦 赵铠 |
| 作者单位： | 100024,北京天坛生物制品股份有限公司乙型肝炎室;300192,天津博荟生物制品有限公司;100024,北京生物制品研究所 |
| 摘 要： | 实时荧光定量PCR(real_time quantitative PCR)技术是一种新型的核酸定量检测、分析技术,它通过在PCR扩增反应过程中加入荧光物质,使得对反应过程的实时监控成为可能。它具有实时监测、定量准确、灵敏度高、反应速度快、重复性好及PCR反应后不需电泳检测等优点,已逐步成为分子生物学研究中的重要工具。 |
| 关 键 词： | 聚合酶链反应 实时定量 荧光 |
| 收稿时间： | 2005-10-24 |
| 修稿时间： | 2005-10-24 |
Progress in real-time quantitative PCR technique |
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| HONG Yun,LI Jin,WANG He-mu,ZHAO Kai. Progress in real-time quantitative PCR technique[J]. International Journal of Epidemiology and Infectious Disease, 2006, 33(3): 161-163,166 | |
| Authors: | HONG Yun LI Jin WANG He-mu ZHAO Kai |
| Affiliation: | Beijing Tiantan Biological Products Company Limited, Beijing 100024, China |
| Abstract: | Real-time quantitative PCR is a newly developed technique for both nucleic acid analysis and quantitative measurement.Because of using fluorescence material in the reaction,the process of the PCR could be well inspected.The merits of the real-time quantitative PCR,such as its good accuracy,quickness and repeatability,enable it as one of the most important tools in the research of molecular biology. |
| Keywords: | PCR Real-time quantitative Fluorescence |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |