| 小鼠巨细胞病毒模型的建立 |
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| 引用本文： | 季育华,朱月秋,陈淑云. 小鼠巨细胞病毒模型的建立[J]. 中华实验和临床病毒学杂志, 1998, 12(1): 58-60 |
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| 作者姓名： | 季育华 朱月秋 陈淑云 |
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| 作者单位： | 上海第二医科大学附属瑞金医院检验科，上海第二医科大学微生物教研室，日本北里大学医学部 |
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| 摘 要： | 目的为了探讨巨细胞病毒的致病机理。方法４周龄Ｂａｌｂ／Ｃ小鼠腹腔内接种小鼠巨细胞病毒（ＭＣＭＶ）。结果导致小鼠急性感染期体重下降，生长迟缓，唾液腺肿胀以至于死亡。唾液腺中检出高滴度感染性病毒（２．０×１０５ＰＦＵ／ｍｌ）。在小鼠３Ｔ３／Ｓｗｉｓａｌｂｉｎｏ细胞单层上形成的空斑清晰，易判断计数、镜检组织切片可见脑神经原细胞胞浆内包涵体。结论小鼠巨细胞病毒模型的建立为抗－ＣＭＶ有效药物的筛选以及对ＣＭＶ感染的预防、治疗提供了资料。
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| 关 键 词： | 巨细胞病毒 包涵体 小鼠模型 空斑形成单位 |
Primary studies on the establishment of cytomegalovirus murine model |
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| Y Ji,Y Zhu,S Chen. Primary studies on the establishment of cytomegalovirus murine model[J]. Chinese journal of experimental and clinical virology, 1998, 12(1): 58-60 |
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| Authors: | Y Ji Y Zhu S Chen |
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| Affiliation: | Department of Clinical Laboratory, Rui Jin Hospital, Shanghai 200025. |
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| Abstract: | Intraperitoneal inoculation of Murine cytomegalovirus(MCMV) into Balb/C mice at age of 4 weeks resulted in acute MCMV infection of the mice. The infected mice had body weight reduction, growth retardation, salivary gland swelling and death. A lethal dose was 5 x 10(6) PFU/ml of MCMV. Virus could be recovered from salivary gland and the infectivity titer of MCMV reached to a high titer of 2 x 10(5) PFU/ml. Plaque formation of MCMV in 3T3/Swiss albino cells was identified. Histopathological examination showed the appearance of MCMV-specific intracytoplasmic inclusion bodies in neuron cells of the mouse brain. |
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| Keywords: | Cytomegalovirus Inclusion body Plaque forming unit Mice models |
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