用固相C_(3b)酶联免疫吸附试验检测经聚乙二醇分离的血清组分中免疫胶固素和循环免疫复合物

本文介绍用3.5％聚乙二醇的沉淀作用预先使IC和血清其他组分分离,再用聚苯乙烯表面吸附的固相C_(3b)从上清液中以抗原抗体反应结合IK,从沉淀复溶液中以受体识别作用结合IC;从而应用C_(3b)—ELISA对同一份血清中IK和IC平行定量检测。临床测定表明,ICD患者血清中IgM类IK检出率为30—45％,IgG类IC为40—57％;且IK和IC双阳性率在半数以上。动态观察发现双阳性的出现和消失与病情的发展和转归有平行关系。本法重复性较好,也较灵敏。所以既适于临床应用,也适用于研究工作。

Use of C3b-ELISA for the Detection of IK and CIC in Serum Separated by PEG Precipitation

The purpose of this work was to detct IK and CIC in the same serum by C_3b-ELISA. It was demonstrated that serum IK and CIC was separated effectively by an initial precipitation step using 3.5% PEG (M. W. 6000). IK contained in the supernatant and IC present in the solubilized precipitate were able to bind specifically to solid-phase C_3b. It was worthy of note that in sera of ICD patients with either positive IK or IC. the percentage of IK and IC double positive results varied in the tests from 45% to 96%. The kinetic observation on patients with IGD suggested that there existed a parallelism between the percentage of double postive and the severity of the disease. C_3b-ELISA is simple, rapid, and inexpensive to perform and it seems to provide a specific, sensitive, and versatile tool for the quantitative analysis of IK and IC.