Livin与EGFR、VEGF在结直肠癌中的表达及相关性研究

【目的】探讨Livin基因在结直肠癌中的表达及其与表皮生长因子受体（EGFR ）、血管内皮生长因子（VEGF）的相关性。【方法】采用免疫组织化学染色方法检测结直肠癌、大肠正常黏膜组织各50例中Livin、EG‐FR、VEGF的表达，并分析Livin表达与EGFR、VEGF的相关性。【结果】结直肠癌组织中Livin蛋白的阳性表达率明显高于大肠正常黏膜组织（ P ＜0．01）；Livin蛋白阳性表达率与淋巴结转移及Duke分期有关，而与结直肠癌患者的年龄、性别、分化程度无关；结直肠癌组织中 EGFR蛋白的阳性表达率明显高于正常黏膜组织（ P ＜0．01）；EGFR阳性表达率与结直肠癌中肿瘤的分化程度、Duke分期及淋巴结转移密切相关，与结直肠癌患者的年龄、性别无统计学意义；大肠正常黏膜组织中VEGF的阳性表达率低于结直肠癌组织中VEGF的阳性表达率（ P ＜0．01），其差异有统计学意义，VEGF蛋白表达与结直肠癌患者的性别、年龄、肿瘤分化程度无关，与肿瘤的Duke分期及有无淋巴结转移有关；在结直肠癌组织中Livin蛋白阳性表达与 EGFR ，VEGF蛋白阳性表达无相关性，而EGFR蛋白阳性表达与VEGF蛋白阳性表达呈正相关。【结论】Livin在结直肠癌组织中过表达，阳性表达率明显高于大肠正常黏膜组织，推测Livin促进了结直肠癌的发生、发展。EGFR、VEGF蛋白在结直肠癌组织中表达呈正相关，推测EGFR、VEGF在结直肠癌的发生发展过程中起着至关重要的作用。

Expression of Livin,EGFR and VEGF in Colorectal Cancer and Their Correlation

Abstract ]Objective]To explore the expression of Livin in colorectal cancer and its correlation with epidermal growth factor receptor（EGFR） and vascular endothelial growth factor （VEGF） .Methods] The expressions of Livin , EGFR and VEGF in 50 cases of colon cancer and 50 cases of normal colorectal mucosa were detected by immunohisto‐chemistry staining .The correlation of Livin with EGFR and VEGF was analyzed .Results] The positive expression rate of Livin in colorectal cancer was obviously higher than that in normal colorectal mucosa （ P〈0 .01） .The positive expres‐sion rate of Livin protein was related to lymph node metastasis and Duke stage ,but not related to the age ,sex and differ‐entiation degree of patients with colorectal cancer .The positive expression rate of VEGF in normal colorectal mucosa was lower than that in colorectal cancer ,and there was significant difference （ P 〈0 .01） .The expression of VEGF protein was not related to the sex ,age and tumor differentiation degree ,but related to Duke stage and lymph node metastasis . The positive expression of Livin protein in colorectal cancer was not related to the positive expression of EGFR and VEGF ,but the positive expression of EGFR was positively correlated with the positive expression of VEGF .Conclusion]Livin is over expressed in colorectal cancer .The positive expression of Livin in colorectal cancer is obviously higher than that in normal colorectal mucosa which infers that Livin promotes the occurrence and development of colorectal cancer . EGFR expression in colorectal cancer is positively correlated with VEGF expression which infers that EGFR and VEGF play very important role in the occurrence and development of colorectal cancer .