递增负荷运动大鼠脑皮质神经元和脑脊液中去甲肾上腺素、5-羟色胺的动态变化*

背景：运动性疲劳的发生、恢复是否与脑脊液循环存在着某种联系？ 目的：观察递增负荷运动后大鼠大脑皮质和脑脊液中去甲肾上腺素、5-羟色胺的动态变化及其相关性。 方法：随机将30只雄性SD大鼠分为对照组10只，运动组20只，运动组采用6周递增负荷运动方案训练，游泳时间由第1周的10 min递增为第6周的60 min，负重由体质量的3%增至体质量的5%。对照组正常饲养。 结果与结论：高效液相色谱检测结果显示，递增负荷运动后即刻，大鼠大脑皮质和脑脊液中去甲肾上腺素、5-羟色胺的水平显著增高；递增负荷运动后24 h，大鼠大脑皮质中去甲肾上腺素、5-羟色胺水平继续增高(P < 0.01)，而脑脊液中去甲肾上腺素、5-羟色胺降至正常水平。其中，递增负荷运动后即刻，大鼠大脑皮质中的去甲肾上腺素与5-羟色胺水平及呈显著正相关(P < 0.05)，大脑皮质和脑脊液中的去甲肾上腺素及5-羟色胺水平高度相关(P < 0.01)。提示递增负荷运动后去甲肾上腺素的兴奋作用和5-羟色胺的抑制作用处于动态平衡中。递增负荷运动后的即刻，脑脊液中去甲肾上腺素、5-羟色胺的变化能够反映大脑皮质中去甲肾上腺素、5-羟色胺的变化。

Dynamic changes of norepinephrine and 5-hydroxytryptamine in cerebral cortex neuron and cerebrospinal fluid of rats after movement with increasing loads

BACKGROUND: Exercise-induced fatigue is a hot topic in the field of sports medicine, and whether there is some association between exercise-induced fatigue occurrence and recovery and cerebrospinal fluid circulation remains poorly understood. OBJECTIVE: To study the dynamic changes and correlation of norepinephrine (NE) and 5-hydroxytryptamine (5-HT) in cerebral cortex and cerebrospinal fluid of rats after movement with increasing loads. METHODS: 30 male SD rats were evenly divided into control group (C), immediately after incremental exercise group (E1), 24 hours after incremental exercise (E2). The control group rats were conventionally fed, and the E1 and E2 group rats received 6 weeks of incremental exercise and then sacrificed immediately and 24 hours after exercise. Cerebrospinal fluid was extracted and cerebral cortex was taken. NE and 5-HT levels in cerebral cortex and cerebrospinal fluid were detected by high-performance liquid chromatography-fluorescence detection. RESULTS AND CONCLUSION: The NE and 5-HT levels in the cerebral cortex and cerebrospinal fluid in the E1 group were significantly higher compared with the C group (P < 0.01). The NE and 5-HT levels in the cerebral cortex were significantly higher in the E2 group compared with the C group (P < 0.01), but there were no significant differences in NE and 5-HT levels in cerebrospinal fluid between the E2 group and the C group. In the E1 group, NE and 5-HT levels in the cerebral cortex were significantly positively correlated (P < 0.05) and NE and 5-HT levels in the cerebral cortex and cerebrospinal fluid were highly correlated (P < 0.01). Results showed that immediately and 24 hours after incremental exercise, the excited effect of NE and the inhibitory effect of 5-HT were in a dynamic balance and that immediately after incremental exercise, the dynamic changes of NE and 5-HT in the cerebrospinal fluid can reflect the dynamic changes of NE and 5-HT in the cerebral cortex.