治伤巴布剂的镇痛作用及对大鼠炎性疼痛模型背根神经节Nav1.7表达的影响

目的：研究治伤巴布剂对大鼠炎性疼痛是否有镇痛作用，探讨治伤巴布剂对福尔马林足底炎性疼痛大鼠模型背根神经节中Nav1.7水平的影响。方法：SD大鼠36只，随机分为3组，即空白对照组、模型组、治疗组，每组12只，空白对照组大鼠不予任何处理，模型组左后足底注射5%福尔马林0.1 mL，治疗组大鼠造模前1天于左后足外敷治伤巴布剂，后左后足底注射5%福尔马林0.1 mL，观察造模后大鼠疼痛行为学反应，并以Dubuisson评分方法进行记录比较。各组大鼠在指定时间点取出L3-6节段背根神经节，运用实时荧光定量、蛋白质印迹检测 Nav1.7在大鼠背根神经节中的表达水平。结果：治疗组大鼠疼痛反应积分低于模型组（P〈0.05﹚；实时荧光定量结果示，模型组Nav1.7mRNA相对表达量大于治疗组，治疗组Nav1.7mRNA相对表达量大于空白组，3组组间比较差异显著（P〈0.05），3组组内3个时相点的相对表达量差异均无统计学意义；蛋白质印迹法灰度扫描示模型组Nav1.7相对表达量大于治疗组，治疗组Nav1.7相对表达量大于空白组，3组组间比较差异显著（P〈0.05），3组组内3个时相点的相对表达量差异均无统计学意义。结论：治伤巴布剂能缓解大鼠炎性疼痛反应，对大鼠炎性疼痛具有一定的镇痛作用，并对福尔马林足底炎性疼痛大鼠模型背根神经节中Nav1.7的表达水平有影响。

Effect of Curing-injury Cataplasma on Analgesia and Expression of Nav1 . 7 in Model Rats with Formaldehyde-induced Inflammatory Pain

This study was aimed to observe the analgesia of curing-injury Cataplasma and discuss the Nav1 . 7 expression in dorsal root ganglion （ DRG ） in model rats with formaldehyde-induced inflammatory pain . A total of 36 Sprague-Dawley rats were divided into three groups, which were the blank control group （n = 12）, model group （ n = 12 ） , and treatment group （ n = 12 ） . The blank control group was without any treatment . The model group was injected with 0 . 1 mL 5% saline formalin on the left rear foot . The treatment group was applied with curing-injury Cataplasma on the left rear foot 24 h before the injection of 0 . 1 mL 5% saline formalin in the establishment of animal model . The behavior reactions to pain of model rats were observed . Dubuisson score was recorded and compared . Meanwhile , L3-6 DRG was collected from rats in each group . The expres-sion of Nav1 . 7 was detected by real-time quantitative PCR and western blot . The results showed that the pain reaction integral in the treatment group was lower than the model group （ P 〈 0 . 05 ） . Results from the real-time quantitative PCR showed that the relative expression of Nav1 . 7 mRNA in the model group was more than the treatment group . And the relative expression of Nav1 . 7 mRNA in the treatment group was more than the blank control group . There was significant difference among three groups （ P 〈 0 . 05 ） . There was no statistical difference at the three time points within three groups. Results from the western blot showed that the relative expression of Nav1 . 7 in the model group was more than the treatment group . And the expression of Nav1 . 7 in＆amp;nbsp;the treatment group was more than the blank control group . There was significant difference among three groups （P 〈 0.05）. There was no statistical difference at the three time points within three groups. It was concluded that the curing-injury Cataplasma can alleviate inflammatory pain response in rats, and have certain analgesia effect . Meanwhile , it can influence Nav1 . 7 expression in DRG in model rats with formaldehyde-induced inflam-matory pain .