小鼠腹腔巨噬细胞清道夫受体活性的影响因素

本实验采用油红Ｏ染色和细胞图像分析法定量研究了ＮＩＨ小鼠腹腔巨噬细胞在不同培养环境下对氧化型低密度脂蛋白（ｏｘｉｄｉｚｅｄｌｏｗｄｅｎｓｉｔｙｌｉｐｏｐｒｏｔｅｉｎ，ＯＬＤＬ）的摄取。结果显示：预先以含５％的牛血清白蛋白的ＲＰＭＩ１６４０无脂培养或５０ｍｇ·Ｌ－１ＯＬＤＬ处理后分别使巨噬细胞摄取ＯＬＤＬ增加了ｇ·ｔ－１的卡介苗处理后分别使ＯＬＤＬ的摄取量下降了４６％和２６％。１００ｍｇ·Ｌ－１的ＳｉＯ２处理后的结果与对照组无显著性差异。表明低脂环境和ＯＬＤＬ对清道夫受体活性的诱导作用，高ＬＤＬ环境和卡介苗抑制清道夫受体活性。

Influencing Factors of Scavenger Receptor Activity or Mouse Peritoneal Macrophages

Oil red O staining and analysis of cell image are used to quantitate and compare the uptake of oxidized low density lipoprotein (OLDL)bymoues peritoneal macrophages under different cultural conditions. The results show that absence of LDL (1640 medium contains 5 percent BSA) and OLDL preincubation enhances the uptake of OLDL by 25 and 105 percent respectively, while high concentration of LDL (1640 medium contains 200 mg. L-1 LDL) or 1 g ·L-1 BCG preincubation decrease the uptake by 46percent and 26 percent. No predminant alteration occurs after pretreatment of 100 mg·L-1 SiO2. The results suggest that absence of LDL and OLDL preincubation induce the scavenger receptor activity, while high concentration of LDL or BCG suppress those receptors.