淫羊藿总黄酮含药血浆对泪腺上皮细胞凋亡模型Caspase-3及Caspase-8表达的影响

目的观察淫羊藿总黄酮含药血浆对雄兔泪腺上皮细胞半胱氨酸蛋白酶-3(Caspase-3)及Caspase-8表达的影响,探究其作用机制。方法将体外培养的雄兔泪腺上皮细胞,随机分为淫羊藿黄酮治疗组(黄酮组)、含雄激素培养对照组(雄激素组)和空白对照组(空白组)。分别加入含淫羊藿总黄酮血浆、丙酸睾酮、空白血浆干预48 h后,各组加入双氧水(H2O2)诱导细胞凋亡。采用Western Blot法检测Caspase-3、Caspase-8的表达情况。结果与空白组比较,黄酮组及雄激素组Caspase-3、Caspase-8蛋白表达降低(P<0.01),同时黄酮组较雄激素组的Caspase-3、Caspase-8蛋白表达降低(P<0.01)。结论淫羊藿总黄酮含药血浆作用于泪腺上皮细胞凋亡模型可抑制Caspase-3及Caspase-8的表达。

Effect of Caspase-3 and Caspase-8 in Apoptosis Model of Lacrimal Gland Epithelial Cells Influenced by Epimedium Flavonoids Drug-Containing Plasma

Objective To observe the effect on expression of Caspase-3 and Caspase-8 in epimedium flavonoids drug-containing plasma on apoptosis model of lacrimal gland epithelial cells,and explore the mechanism.Methods The lacrimal gland epithelial cells were randomly divided into the epimedium flavonoids group,the control group with androgen cultural(androgen group),and the blank group.Total flavonoids with epimedium plasma,testosterone propionate and blank plasmain were added for 48 h after intervention respectively.Then,the apoptosis model was established by H2O2.Western Blot was used to detect the expression of Caspase-3 and Caspase-8 in lacrimal gland epithelial cells.Results Compared with the blank group,the protein expressions of Caspase-3 and Caspase-8 decreased in the epimedium flavonoids group and the androgen group(P<0.01).Meanwhile,the protein expressions of Caspase-3 and Caspase-8 in the epimedium flavonoids group were lower than those in the androgen group(P<0.01).Conclusion Epimedium flavonoids drug-containing plasma can inhibit the expression of Caspase-3 and Caspase-8 in apoptosis model of lacrimal gland epithelial cells.