玻璃表面可控聚乙二醇生物活性涂层构建及其血液相容性研究

运用表面引发原子转移自由基聚合反应(SI-ATRP)在玻璃表面构建甲基丙烯酸聚乙二醇酯(PEGMA)-甲基丙烯酸缩水甘油酯(GMA)的二元嵌段共聚物(PEGMA-GMA),然后利用GMA中丰富的环氧基团将内皮细胞选择性多肽精氨酸-谷氨酸-天冬氨酸-缬氨酸(REDV)通过开环反应固定在PEGMA-GMA聚合物刷的末端。采用静态水接触角、X射线光电子能谱(XPS)以及原子力显微镜(AFM)等对聚合物刷的结构和亲水性能进行了表征,结果证明在玻璃基材表面成功构建了REDV多肽修饰的二元嵌段共聚物刷;同时利用紫外-可见吸收光谱(UV-Vis)对表面固定的REDV进行了定量表征。最后采用复钙化凝血时间和血小板黏附实验对涂层的血液相容性进行表征,结果显示聚合物涂层具有良好的血液相容性。这种修饰多肽的聚乙二醇生物活性涂层为后续表面内皮化研究奠定了良好的前期基础。

Construction of controllable polyethylene glycol bioactive coating with hemocompatibility from the surface of modified glass substrate

A diblock copolymer,poly(ethylene glycol)methacrylate-block-glycidyl methacrylate(PEGMA-GMA),was prepared on glass substrate by surface-initiated atom transfer radical polymerization(SI-ATRP),and endothelial specific peptide Arg-Glu-Asp-Val(REDV)was immobilized at the end of the PEGMA-GMA polymer brush by ring opening reaction through the rich epoxy groups in the GMA.The structure and hydrophilicity of the polymer brushes were characterized by static water contact angle,X-ray photoelectron spectroscopy(XPS)and atomic force microscopy(AFM).The results showed that the REDV modified copolymer brushes were successfully constructed on the glass substrates.The REDV peptide immobilized onto surface was quantitatively characterized by ultraviolet–visible spectroscopy(UV-VIS).The blood compatibility of the coating was characterized by recalcification time and platelet adhesion assay.The results showed that the polymer coating had good blood compatibility.The multifunctional active polymer coating with PEGMA and peptide produced an excellent prospect in surface construction with endothelial cells selectivity.