模拟微重力环境下核因子κB信号通路调节MC3T3-E1细胞成骨分化的实验研究

本文旨在研究微重力环境对MC3T3-E1细胞成骨分化的影响,并进一步探讨微重力环境下核因子κB(NF-κB)信号通路对成骨细胞零重力的响应机制。将MC3T3-E1细胞分别进行正常培养(CON)和模拟微重力培养(SMG),培养期满后观察成骨性相关分子表达变化以及NF-κB信号通路变化情况。结果显示,在微重力条件下,碱性磷酸酶(ALP)、骨钙素(OCN)、一型胶原(CoL-Ⅰ)的基因和蛋白水平表达下调;与此同时,NF-κB信号抑制子α(IκB-α)下调,p65表达显著上调,且二者磷酸化水平升高,细胞培养液中IL-6含量增多。研究表明,微重力环境下NF-κB信号通路可激活并调控成骨细胞分化。

Research of simulated microgravity regulate MC3T3-E1 cells differentiation through the nuclear factor-kappa B signaling pathway

In this study,we aim to investigat the effect of microgravity on osteoblast differentiation in osteoblastlike cells(MC3T3-E1).In addition,we explored the response mechanism of nuclear factor-kappa B(NF-κB)signaling pathway to“zero-g”in MC3T3-E1 cells under the simulated microgravity conditions.MC3T3-E1 were cultured in conventional(CON)and simulated microgravity(SMG),respectively.Then,the expression of the related osteoblastic genes and the specific molecules in NF-κB signaling pathway were measured.The results showed that the mRNA and protein levels of alkaline phosphatase(ALP),osteocalcin(OCN)and typeⅠcollagen(CoL-Ⅰ)were dramatically decreased under the simulated microgravity.Meanwhile,the NF-κB inhibitorα(IκB-α)protein level was decreased and the expressions of phosphorylation of IκB-α(p-IκB-α),p65 and phosphorylation of p65(p-p65)were significantly upregulated in SMG group.In addition,the IL-6 content in SMG group was increased compared to CON.These results indicated that simulated microgravity could activate the NF-κB pathway to regulate MC3T3-E1 cells differentiation.