外周血淋巴细胞白细胞介素-2 mRNA监测在肾移植术后早期急性排斥反应诊断中的应用

Objective To study the application of peripheral blood lymphocyte (PBL) interleukin (IL) -2 mRNA evaluation with real-time polymerase chain reaction ( PCR) for the early acute rejection (AR) diagnosis of patients with renal transplantation. Methods For 68 renal recipients from May 2008 to August 2009, PBL IL-2 mRNA expression was detected before and 1,3,7, 14 days after operation. All patients were divided into AR group and control group. Results On the postoperative day 6 to 20, AR occurred in 9 cases. Before operation and 1, and 3 days after operation, PBL IL-2 mRNA expression in AR group and control group was (0. 182 ±0. 230) and (0.176 ±0. 015), (0. 174 ±0. 018) and (0. 168 ± 0. 023) (P > 0.05 ). On the postoperative day 7, PBL IL-2 mRNA expression in AR group and control group was (0. 270 ± 0.056) and (0. 195 ± 0.053 ) respectively (P < 0. 05). On the postoperative day 14, PBL IL-2 mRNA expression in AR group and control group was (0. 320 ±0. 043) and (0. 181 ±0. 034) respectively ( P < 0.01). Conclusion PBL IL-2 mRNA evaluation with real-time PCR is the effective method for monitoring early AR on renal recipients, and is beneficial to the timely treatment and the prognostic decision.

Application of peripheral blood lymphocyte IL-2 mRNA evaluation in early acute rejection diagnosis of patients with renal transplantation