SLE患者T细胞亚群及CD4+CD25+T细胞的检测及意义

目的探讨T淋巴细胞亚群及CD4 CD25 T细胞在SLE病人发病中的临床意义。方法采用流式细胞术检测了68例SLE患者(其中活动期病人38例,稳定期病人30例)以及30例健康体检者外周血CD3 CD4 、CD3 CD8 、CD4 CD45RA 、CD8 CD28 、CD8 CD28-T细胞亚群以及CD4 CD25 T细胞水平。结果与正常对照组相比,活动期SLE病人CD3 CD4 、CD4 CD45RA 细胞亚群的百分率明显降低(P=0.000,P=0.001),CD3 CD8 、CD8 CD28-细胞亚群的百分率明显升高(P=0.000,P=0.000)。稳定期患者CD8 CD28 细胞水平明显高于活动期和对照组(P=0.011,P=0.435),CD3 CD4 、CD4 CD45RA 水平高于活动期,但无显著性差异(P=0.067,P=0.081),CD8 CD28-T细胞亚群无明显变化(P=0.997)。活动期病人CD4 CD25 T细胞百分率明显低于正常对照组及稳定期病人(P=0.000,P=0.000),稳定期病人与对照组之间无显著性差异(P=0.572)。结论SLE患者T淋巴细胞亚群的水平是异常的。病人外周血CD8 CD28-T细胞亚群的比例升高与疾病的病程和临床表现相关联,它的升高在疾病的稳定中起重要作用。CD4 CD25 T细胞水平降低可能是导致机体抑制自身免疫反应的功能减弱并引发SLE发生发展的重要因素。

Clinical Significance of T Cell Subsets and CD4+CD25+ T cells of SLE Patients

Objective To study the clinical significance of the percentages of T cell subsets and CD4 CD25 regulatory T cells of peripheral blood in SLE patients. Methods The percentages of CD3 CD4 , CD3 CD8 , CD4 CD45RA ,CD8 CD28 ,CD8 CD28- T cell subsets and CD4 CD25 regulatory T cells of peripheral blood from 68 SLE patients (38 in active stage and 30 in stable stage) and 30 normal subjects (control group) were determined by flow cytometry. Results Compared with control group, the percentages of CD3 CD4 and CD4 CD45RA T cell subsets of SLE patients in active stage were decreased (P=0.000,P=0.001), while the percentages of CD3 CD8 and CD8 CD28- T cell subsets were significantly increased(P=0.000,P=0.000). The level of CD8 CD28 T cell subsets of SLE patients in stable stage were higher than that in active stage and control group(P=0.011,P=0.435). The levels of CD3 CD4 and CD4 CD45RA of SLE patients in stable stage were higher than that inactive stage, but there is no difference between these two subsets (P=0.067, P=0.081).The level of CD4 CD25 regulatory T cells was significantly decreased in patients with disease at active stage when compared with control group and patients with disease at stable stage (P=0.000,P=0.001). No difference was observed between patients with disease at stable stage and healthy subjects(P=0.572. Conclusion T cell subsets of SLE patients are abnormal. The increase of CD8 CD28- T cells in SLE patients is related to disease stage and clinical manifestation and may play a major role in stabilizing the condition of SLE patients. The decrease of CD4 CD25 T cells may lead to the weakening of suppressing auto-reactive immune responses and play a vital role in the pathogeneses of SLE.