肝癌组织及外周血端粒酶表达对肝癌的诊断和鉴别价值

目的　探讨肝癌组织端粒酶表达及外周血端粒酶分析对肝癌的诊断价值。方法　从人肝癌的癌灶和癌周组织制备总RNA ,肝组织及外周血单核细胞端粒酶活性 ,采用端粒重复扩增法 酶联免疫吸附试验分析 ,并观察了外周血中端粒酶活性对肝癌的诊断与鉴别诊断价值。结果　肝癌组织中端粒酶活性表达明显高于它的癌周组织 ,而总RNA则是癌周组织明显高于癌灶 ;在外周血单核细胞中 ,未经治疗的肝癌组端粒酶活性明显高于肝硬化组、慢性肝炎组及正常对照组 (P <0 0 1) ,经过TAE治疗后肝癌病人端粒酶活性明显下降 (P <0 0 1) ;外周血AFP浓度与单核细胞端粒酶活性联合分析 ,对肝癌具有互补诊断价值。结论　肝癌发生过程中端粒酶表达异常 ,分析外周血中端粒酶活性有助于肝癌的诊断及预后观察

Expression of telomerase in hepatoma tissues and peripheral blood and its clinical implications in differentiation diagnosis of hepatocelluar carcinoma

Objective To explore the expression of telomerase in hepatoma and clinical implications of peripheral blood mononuclear cell telomerase activity in patients with hepatocellular carcinoma (HCC).Methods Total RNAs were extracted from hepatomas and their distal cancerous tissues, the telomerase activities of livers and peripheral blood mononuclear cells ( PBMCs) were analyzed by TRAP-ELISA,and its clinical values were investigated in diagnosis and differentiation of HCC.Results The expression of telomerase in hepatomas were significantly higher than that in their distal cancerous tissues, and total RNA levels were lower in former than that in latter. The telomerases of PBMCs in untreated HCC were much higher than those in liver cirrhosis, chronic hepatitis and control group (p<0.01), respectively.Obviously decrese of telomerase in HCC was found in TAE treated group than that in untreated group (p<0.01). The combination of serum AFP and telomerases of PBMCs were more sensitivity and specificity for HCC diagnosis.Conclusions The present data suggested that the telomerase in HCC tissues were overexpressed and circulating telomerase could be a useful marker to diagnosis and prognosis of HCC.