荧光定量聚合酶链反应诊断结核病临床应用研究

目的探讨荧光定量聚合酶链反应（FQ-PCR）技术诊断结核病临床应用价值。方法应用FQ-PCR技术检测患者痰、外周血、体液（包括各种渗出液及脑脊液）中结核分枝杆菌DNA（TB-DNA），并与定性PCR、常规细菌学涂片法和培养法比较。结果112例结核病患者FQ-PCR、定性PCR、涂片法和培养法检测各类标本阳性率为59．82％，88．82％，25．89％，29．46％，非结核组43例FQ-PCR法阳性率是2．32％，PCR法是18．60％，FQ-PCR检测其标本TB-DNA的特异性为97．67％，敏感度为59．82％，阳性预测值为98．53％。结论FQ-PCR检测TB-DNA是一种快速简便、敏感性、特异性、可信性较高的方法，对诊断结核病具有临床意义，且适用于临床检验实验室，其含量变化对判断疗效有一定的提示作用。

Diagnosis of tuberculosis by fluorescence quantities polymerase chain reaction

Objective To evaluate the clinical value of fluorescence quantitative PCR(FQ-PCR) technique in diagnosing tuberculosis.Methods FQ-PCR was uesd to detect the tuberculosis DNA(TB-DNA) from 112 tuberculosis patients.The result was compared with qualitative PCR,routine bacteriological smear,and culture methods.Results The positive rates of FQ-PCR,qualitative PCR,routine bacterological smear,and culture were 59.8%,84.82%,25.89%,and 29.46%,respectively.The FQ-PCR and PCR positive rates of 43 patients without tuberculosis were 2.32% and 18.60%.The FQ-PCR specificity and sensitivity were 97.53% and 59.82%,and its positive predictive vita was 98.53%.Conclusion FQ-PCR for detecting TB-DNA is rapid and simple,and has higher sensitivity,specificity and reliability,and is of clinic practical significance for diagnosing.