全反式维甲酸和三氧化二砷抑制子宫内膜癌细胞生长及其与NDRG1基因的关系

目的 探讨全反式维甲酸（ATRA）和三氧化二砷（As2O3）对人子宫内膜癌HEC-2B细胞体外生长的抑制作用及其与分化相关基因1（NDRG1）的关系。方法 采用MTT法比较不同浓度（1×10-7、5×10-7、1×10-6、5×10-6和1×10-5mol／L）ATRA和（1.0、2.0、4.0、8.0和16.0μmol／L）As2O3对子宫内膜癌HEC-2B细胞的增殖抑制作用，Western blotting检测HEC-2B细胞中NDRG1蛋白的表达情况。结果 镜下观察，经ATRA和As2O3作用48h后的子宫内膜癌HEC-2B细胞呈现凋亡的特征性改变。1×10-7～1×10-5mol／L 的ATRA和1.0～16.0μmol／L的As2O3能够抑制子宫内膜癌HEC-2B细胞的生长，呈剂量和时间依赖性。ATRA和As2O3能够从蛋白水平调节NDRG1的表达，其表达随药物浓度的增加逐渐下调。结论 ATRA和As2O3能够抑制子宫内膜癌HEC-2B细胞的生长，该抑制作用可能与NDRG1蛋白下调有关。

The inhibitory effect of all-trans retinoic acid and arsenic trioxide on the growth of endometrial cancer cells and the relation with NDRG1

Objective To explore the inhibitory effect of all-traMs retinoic acid（ATRA） and arsenic trioxide（ As2O3 ） on the growth of human endometrial cancer HEC-2B cells in vitro and its relation with N-myc downstream regulated genel （ NDRG1 ） protein. Methods Tetrazolium salt assay （MTT） was used to compare the inhibitory effect of different concentration of ATRA （ 1 ×10^-7, 5 ×10^-7, 1 ×10^-6, 5 ×10^-6 and 1 × 10^-5 mol/L） and As2O3（1.0, 2.0, 4.0, 8.0 and 16.01μmol/L） on the growth of HEC-2B cells. The expression of NDRG1 protein was determined by Western blotting analysis. Results After treated with ATRA and As203 for 48h, the HEC-2B cells showed apoptotic change. ATRA 1 × 10^-7-1 ×10^5mol/L and As2O3 1.0-16. 0μmol/L inhibited the cell prolifera- tion in a dose-and time-dependent manner. Western blotting method showed that ATRA and As2O3down-regulated the expression of NDRG1 protein in a dose-dependent manner. Conclusion ATRA and As2O3 can significantly inhibit the growth of human endometrial cancer HEC-2B cells in vitro. The effect may be related to the down-regulation of NDRG1 protein expression.