PTEN、nm23-H1和Syk在大肠癌中的表达及意义

目的 检测PTEN、nm23-H1和Syk蛋白在大肠癌中的表达，探讨其与大肠癌发生、发展、浸润和转移的关系。方法 采用免疫组化SP法检测60例大肠癌组织及30例正常大肠黏膜中PTEN、nm23-H1和Syk的表达，分析三者与大肠癌临床病理特征的关系。结果 正常大肠黏膜中PTEN、nm23-H1和Syk的阳性表达率分别为90.0％（27／30）、93.3％（28／30）和96.7％（29／30），而在大肠癌组织中分别为36.7％（22／60）、41.7％（25／60）和31.7％（19／60），差异均有统计学意义（P＜0.01）。大肠癌中3种蛋白的表达与年龄、性别、肿瘤大小和发生部位均无关（P＞0.05），PTEN、Syk表达与大肠癌的浸润程度、淋巴结转移、分化程度及Duke's分期有关，nm23-H1表达与浸润程度、淋巴结转移及Duke's分期有关。大肠癌中3种蛋白表达两两呈正相关。结论 PTEN、nm23-H1和Syk表达与大肠癌发生、发展、浸润及转移密切相关，联合检测是判断大肠癌生物学行为的重要指标。

Expressions and significances of PTEN, nm23-H1 and Syk in colorectal cancer

Objective To evaluate the expressions of PTEN, nm23-H1 and Syk in colorectal cancer and their relationships with the carcinogenesis, development, invasion and metastasis of colorectal cancer. Methods The expression of PTEN, nm23-H1 and Syk proteins in 60 cancer tissues and 30 normal colorectal mucous tissues were determined by the immunohistochemieal SP method. Their correlations with clinicopathologic features were analyzed. Results The positive rates of PTEN, nm23-H1 and Syk expression was 90. 0% （27/30） ,93.3% （28/30） and 96. 7% （29/30） in normal tissues, higher than 36. 7% （22/60） ,41.7% （25/60） and 31.7% （19/60） in colorectal cancer tissues, respectively（ P 〈 0. 01 ）. The expression of the three proteins in coloreetal cancer tissues were not associated with age, gender, tumor size or tumor location（ P 〉 0.05 ）. The expressions of PTEN and Syk were significantly re- lated with differentiation, depth of invasion, lymph node metastasis and Duke＇s staging. And the expression of nm23-H1 was associated with depth of invasion, lymph node metastasis and Duke＇s staging. The expression of the three proteins in colorectal cancer tissues were possitively related. Conclusion The expressions of PTEN, nm23-H1 and Syk in colorectal cancer are related to carcinogenesis, devel- opment, invasion and metastasis. The joint detection of these three makers can be used to determine the biological behavior and prog- nosis of colorectal cancer.