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| 肝素结合生长因子的纯化及其刺激人血管内皮细胞增生作用的研究 | |
| 引用本文： | 徐雍,杨平阿,沈静,李纳新,李传余,王天保. 肝素结合生长因子的纯化及其刺激人血管内皮细胞增生作用的研究[J]. 中国病理生理杂志, 1992, 0(4) |
| 作者姓名： | 徐雍 杨平阿 沈静 李纳新 李传余 王天保 |
| 作者单位： | 北京友谊医院临床医学研究所病理生理研究室(徐雍，杨平阿，沈静，李纳新，李传余)，北京友谊医院临床医学研究所病理生理研究室(王天保) |
| 摘 要： | 用40—80%饱和度硫酸铵沉淀、CM-葡聚糖C-50离子交换层析及肝素琼脂糖亲和层析从牛脑中纯化了肝素结合生长因子,SDS-PAGE显示单一带型,分子量约为16kD;以人脐带静脉内皮细胞为靶细胞,对肝素结合生长因子刺激内皮细胞增生作用进行了研究,结果表明,细胞培养时加入肝素结合生长因子,可使内皮细胞长期传代,冷冻后可成功复苏,培养所需血清浓度可降至10—15%。 |
| 关 键 词： | 肝素结合生长因子 层析法 人内皮细胞 |
Purification of HBGFs from bovine brain and its ability to stimulate endothelial cells proliferation |
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| Xu Yong,Yang Ping-A,et al. Purification of HBGFs from bovine brain and its ability to stimulate endothelial cells proliferation[J]. Chinese Journal of Pathophysiology, 1992, 0(4) | |
| Authors: | Xu Yong Yang Ping-A et al |
| Affiliation: | Xu Yong,Yang Ping-A,et al Department of pathophysiology,Institute of Clinical Medicine Beijing Friendship Hospital,Beijing |
| Abstract: | Purification of HBGFs from bovine brain through three steps: 40 to 80% saturated ammonium sulfate fractionatton of bovine brain extract, CM-Sephadex C-50 ionexchange chromatographe, and heparin affinity chromatography. SDS-PAGE revealed the presence of a single band a with molecular weights about 16 kilodalton. The ability of HBGFs to stimulate cell proliferation in vitro was studied using human umbilical vein EC as target cells. The result showed that when HBGFs was added to the cultures, EC could be made to long-term serial cultivation, and resuscitated successfully after freezing and the serum requirement for EC growth could be reduced. |
| Keywords: | Heparin-binding growth factors Chromatography Human endothelial cell |
| 本文献已被 CNKI 等数据库收录！ | |