酶联荧光分析法在血清催乳素测定中的应用及其价值

①目的探讨酶联荧光分析法(ELFA)测定血清催乳素及其临床应用价值。②方法采用ELFA法测定不同浓度血清催乳素(PRL)的精密度、灵敏度、线性范围、回收率、标准曲线的稳定性及干扰因素。③结果ELFA法测定PRL的总CV值为3.2%~4.3%;分析灵敏度为0.5ng/mL,线性范围为0.5~200ng/mL,回收率为102.2%,标准曲线的稳定性为12天,高浓度的胆红素、血红蛋白和甘油三酯对对测定结果无显著性影响(P>0.05)。④结论ELFA法测定血清PRL操作简便,结果准确可靠,自动化程度高,且检测速度快,有较宽的线性范围和较高的精密度。

Evaluation of prolactin in serum by enzyme - linked fluorescent assay and its clinical application

Objective To evaluate the analytical features of the new automated prolactin assay using enzyme-linked fluorescent assay(ELFA) technology and to obtain information about its utility in clinical studies.Methods The parameters links precision,analytical sensitivity,linearity range,recovery rate,calibration curve stability and interfere factors of the PRL assay were determined.the comparison study was also performed versus enzyme-linked immunosorbent assay(ELISA).Results The total coefficient of variation was from 3.1%~4.3%,the minimal detection limit was 0.5ng/ml,the linearity range was from 0.5~200ng/mL,the mean recovery rate of dilution samples was 102.2%,the calibration curve was stable for at least 12 days.Adding of high concentration of bilirubin,triglyceride or hemoglobin into samples did not interfere prolactin measurement by proposed method(P>0.05).Conclusion The ELFA method is reliable,precise,fairly rapid and easy to perform.This method is very useful and suitable for routine determinations of serum PRL levels in clinical laboratory.