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| mCD99L2基因沉默对小鼠B淋巴瘤细胞A20生物学特性的影响 | |
| 引用本文： | 陈小艳,刘芳,沈丽佳,李慧灵,张弓,黄作平,王辛,赵彤. mCD99L2基因沉默对小鼠B淋巴瘤细胞A20生物学特性的影响[J]. 热带医学杂志, 2009, 9(2) |
| 作者姓名： | 陈小艳 刘芳 沈丽佳 李慧灵 张弓 黄作平 王辛 赵彤 |
| 作者单位： | 1. 南方医科大学南方医院病理科,广州,510515 2. 广东省分子肿瘤病理学重点实验室,广州,510515 3. 南方医科大学南方医院病理科,广州,510515;广东省分子肿瘤病理学重点实验室,广州,510515 |
| 基金项目： | 国家自然科学基金(No.30670812) |
| 摘 要： | 目的探究mCD99L2基因沉默对小鼠B淋巴瘤细胞系A20细胞生物学特性的影响。方法采用MTT法和流式细胞仪检测mCD99L2基因沉默前后干扰组A20-LV-mCD99L2和未干扰组A20细胞的增殖率和细胞周期;Transwell法测定两组细胞的体外侵袭能力;采用裸鼠与BALB/c鼠皮下成瘤实验观察比较两组细胞在体内的成瘤时间和成瘤率。结果MTT法显示干扰组A20-LV-mCD99L2增殖率为(0.61±0.12),低于未干扰组增殖率(0.75±0.20),差异有统计学意义(P=0.000);干扰组A20-LV-mCD99L2处于G2期的细胞比例为(10.58±4.97),高于未干扰组(3.33±1.31),差异有统计学意义(P=0.009);Transwell法显示干扰组A20-LV-mCD99L2运动能力较未干扰组下降。裸鼠皮下成瘤时间干扰组(13.33±4.63)d长于未干扰组(9.50±2.90)d,成瘤率均为100%;BALB/c鼠皮下成瘤时间干扰组(10d)长于未干扰组(7.0±0.82)d,成瘤率干扰组为14.3%,显著低于未干扰组(100%),差异有统计学意义(P=0.000)。结论mCD99L... |
| 关 键 词： | mCD99L2 基因沉默 干扰 A20细胞 A20-LV-mCD99L2细胞 |
The Influence of mCD99L2 Gene Silencing on the Biological Feature of Mouse B Lymphoma Cell Line A20 |
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| CHEN Xiao-yan,LIU Fang,SHEN Li-jia,LI Hui-ling,ZHANG Gong,HUANG Zuo-ping,WANG Xin,ZHAO Tong. The Influence of mCD99L2 Gene Silencing on the Biological Feature of Mouse B Lymphoma Cell Line A20[J]. Journal Of Tropical Medicine, 2009, 9(2) | |
| Authors: | CHEN Xiao-yan LIU Fang SHEN Li-jia LI Hui-ling ZHANG Gong HUANG Zuo-ping WANG Xin ZHAO Tong |
| Affiliation: | 1. Department of Pathology;Nanfang Hospital;Southern Medical University;Guangzhou 510515;2. Tumor Institute;China |
| Abstract: | Objective To investigate the influence of mCD99L2 gene silencing on the biological feature of mouse B lymphoma cell line A20. Method The cell proliferation rate and cell cycle of SiRNA group A20-LV-mCD99L2 cells and control group A20 cells were detected by MTT and FCM method. The invasive potential of the two groups was determined by Transwell. The tumor formation time and rate of the two groups in vivo were observed by subcutaneous transplantation on nude mice and BALB / c mice. Result The cell proliferati... |
| Keywords: | mCD99L2 gene silencing interfere A20 cell line A20-LV-mCD99L2 cells |
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