骨组织工程材料多肽修饰涂层在骨质疏松中的应用

背景：将具有特定功能的蛋白质、酶、多肽等生物活性物质固定于种植体表面，可促进骨潜能细胞及成骨细胞的增殖、分化，达到良好的成骨作用。目的：介绍生物活性多肽精氨酸-甘氨酸-天冬氨酸的生物学特性，及其在骨质疏松状态下对细胞生物学行为和种植体骨整合的影响。方法：计算机检索PubMed数据库(1984年1月至2013年1月)、万方数据(2002年1月至2012年12月)、CHKD期刊全文数据库(2002年1月至2012年12月)中，有关生物活性多肽精氨酸-甘氨酸-天冬氨酸的生物学特性，以及其在骨质疏松状态下对细胞生物学行为和种植体骨整合影响的文献。结果与结论：在种植体表面接种生物活性物质可进一步改善种植体与骨组织的整合速度。通过在生物材料表面固定精氨酸-甘氨酸-天冬氨酸多肽的方法进行仿生态化学修饰，模拟体内细胞外基质环境，促进细胞黏附和生长，是早期改善内植物骨整合的有效途径，也是近年组织工程领域研究的重要方向。今后研究的重点在于设计特异高效的多肽和简单有效的固定方法，同时将对这类物质长期应用的安全性做进一步深入的研究。

Polypeptide modified coating,a bone tissue engineering material,is used for treatment of osteoporosis

BACKGROUND:The biological substances fixed onto the implant surface, including specific proteins, enzymes, polypeptides, could promote the proliferation and differentiation of osteogenic cells in order to achieve a good osteogenic effect. OBJECTIVE:To introduce the biological characteristics of the arginine-glycine-aspartic acid (RGD) peptides, cell biological behavior and implant osseointegration in osteoporosis. METHODS:Authors retrieved the relevant articles in PubMed database (January 1984 to January 2013), Wanfang database (January 2002 to December 2012) and CHKD database (January 2002 to December 2012) using the key words “RGD peptides, osteoporosis, bone marrow mesenchymal stem cells, osseointegration, osteoblast, induced differentiation”. Papers were included concerning the biological characteristics of RGD peptides, the biological behavior of cells and the bone-implant integration in osteoporosis. RESULTS AND CONCLUSION:Osseointegration on the surface between implant and bone tissue can be further  improved by grafting biologically active substance. Bionics modification, to simulate the extracellular matrix environment in vivo, can promote cell adhesion and growth, which is an effective way to improve osseointegration and is also an important direction of research in the field of tissue engineering recently. The future research is focused on designing specific and efficient polypeptides and simple and effective fixation methods.