胶质细胞源性神经营养因子对猴骨髓间充质干细胞分化为神经元样细胞的影响

背景：鉴于骨髓间充质干细胞体外分化为神经样细胞的最终研究目的,是将诱导后的细胞移植入体内参与损伤神经系统的修复过程,因此,保证移植细胞的活性显得十分重要。目的：探讨胶质细胞源性神经营养因子对隐丹参酮体外诱导猴骨髓间充质干细胞分化为神经元样细胞的保护作用。方法：以隐丹参酮为诱导剂诱导第8代猴骨髓间充质干细胞分化为神经元样细胞,应用流式细胞仪检测不同时段诱导细胞的凋亡百分比(每间隔0.5 h为1组,共12组)。选择细胞凋亡百分比较高的一个时段,观察添加不同质量浓度胶质细胞源性神经营养因子(0-100 μg/L,共11组)对诱导细胞凋亡的影响。结果与结论：诱导后细胞凋亡百分比逐渐升高,约4 h时达到峰值,维持约1 h后下降(P < 0.05)。 随着胶质细胞源性神经营养因子质量浓度由0 μg/L提高到30 μg/L,细胞凋亡百分比逐渐下降(P < 0.05),当胶质细胞源性神经营养因子质量浓度超过30 μg/L后,细胞凋亡水平受胶质细胞源性神经营养因子质量浓度影响不再显著。结果可见胶质细胞源性神经营养因子在隐丹参酮体外诱导猴骨髓间充质干细胞分化为神经元样细胞过程中具有保护作用。

Glial cell line-derived neurotrophic factor promotes neuron-like cell differentiation of mesenchymal stem cells

BACKGROUND:The ultimate purpose for inducing the bone marrow mesenchymal stem cells to differentiate into neuron-like cells in vitro is to transplant the induced cells into the body and repair the damaged nervous system. So, it is important to ensure the activity of the transplanted cells.OBJECTIVE:To investigate the protective effect of glial cell line-derived neurotrophic factor on neuron-like cells differentiated from mucaca multta bone marrow mesenchymal stem cells induced with cryptotanshinone in vitro.METHODS:The passage 8 mucaca multta bone marrow mesenchymal stem cells were induced with cryptotanshinone to differentiate into neuron-like cells, and the percentage of apoptosis of the induced cells at different time points after induction was detected with flow cytometry (0.5 hour was one group, a total of 12 hours). The period with higher apoptosis percentage was selected to observe the effect of different concentrations of glial cell line-derived neurotrophic factor (0-100 μg/L, totally eleven groups) on the apoptosis of the induced cells.RESULTS AND CONCLUSION:Cell apoptosis percentage after induction was increased gradually over time and reached a peak about 4 hours, and decreased after 1 hour (P < 0.05). Cell apoptosis percentage was decreased gradually when the concentration of glial cell line-derived neurotrophic factor increased from 0 μg/L to 30 μg/L (P < 0.05), and more remarkable change were not found when glial cell line-derived neurotrophic factor concentration reached 30 μg/L. The results show that glial cell line-derived neurotrophic factor has the protective effect on the neuron-like cells differentiated from mucaca multta bone marrow mesenchymal stem cells induced with cryptotanshinone in vitro.