芍药苷促椎间盘纤维环细胞增殖的作用

背景：芍药在颈椎病治疗中使用频率超过30%,其主要单体之一芍药苷具有抗炎、抗凋亡等作用,芍药苷对椎间盘纤维环细胞是否有保护作用,国内外未见报道。 目的：探讨芍药苷对椎间盘纤维环细胞的增殖和保护作用。 方法：采用酶消化法体外培养椎间盘纤维环细胞。取第3代纤维环细胞,培养过程中加入20 μg/L Fas配体诱导建立纤维环细胞凋亡模型,通过MTT法测定芍药苷干预纤维环细胞的最适宜浓度,Annexin V/FITC-PI法观察芍药苷对椎间盘纤维环细胞的保护作用。 结果与结论：芍药苷浓度20.8和2.08 μmol/L对椎间盘纤维环细胞具有明显的促增殖作用。20 μg/L Fas配体能诱导细胞凋亡,20.8 μmol/L芍药苷对Fas配体诱导椎间盘纤维环细胞有保护作用。

Paeoniflorin accelerates annulus fibrosus cells proliferation

BACKGROUND:Chinese herbaceous peony has been usually used in the treatment of cervical spondylosis in a frequency of over 30%. Paeoniflorin, known as one of the main monomers, has cell-protective abilities against inflammation and apoptosis. Despite these compelling observations, whether paeoniflorin protects annulus fibrosus cells is not reported. OBJECTIVE:To explore the proliferative and protective effects of paeoniflorin on intervertebral disc annulus fibrosus cells. METHODS:Annulus fibrosus cells derived from the intervertebral disc were digested with trypsin and collagenase type Ⅱ for the further culture in vitro. The third-passage annulus fibrosus cells were selected to establish an apoptosis model in response to Fas ligand (20 μg/L) and were interfered with paeoniflorin. The appropriate concentration of paeoniflorin was detected by the method of MTT. Apoptotic rate was evaluated with Annexin V/FITC double staining. RESULTS AND CONCLUSION:The 20.8 and 2.08 μmol/L paeoniflorin significantly promoted the proliferation of annulus fibrosus cells. The apoptotic rate of annulus fibrosus cells in vitro was significantly increased after induction with the 20 μg/L Fas ligand. 20.8 μmol/L paeoniflorin could protect annulus fibrosus cells against Fas ligand-induced apoptosis.