富集自体外周血单个核细胞移植治疗肝硬化

背景：与骨髓移植相比较，外周血干细胞移植有其自身的优点，外周血中干细胞资源多、收集方便易行、无需麻醉、创伤小、易被患者接受、安全性高、患者的造血系统易恢复等。目的：观察自体外周血单个核细胞对失代偿期肝硬化患者的治疗作用及安全性。方法：选择2010年11月至2011年7月于大连医科大学附属第一医院住院的4例失代偿期肝硬化患者，其中乙型肝炎后失代偿期肝硬化3例，自身免疫性肝病后失代偿期肝硬化1例，年龄31-67岁，平均年龄44岁。经粒细胞集落刺激因子动员后采集外周血干细胞，行肝动脉插管自体外周血单个核细胞移植治疗。结果与结论：外周血单个核细胞采集、肝动脉内干细胞移植过程中及移植后无发热、出血、恶心等不良反应发生，行自体外周血单个核细胞移植第 1，3，6个月后，纳差、乏力、食欲、腹胀等临床症状得到不同程度的改善。实验室检测肝功能和肝纤维化指标得到一定程度的改善。

Peripheral blood mononuclear cell transplantation for liver cirrhosis

BACKGROUND: Compared with bone marrow transplantation, peripheral blood stem cell transplantation has its own advantages, including rich resources of stem cells from the peripheral blood, convenient and easy collection, without anesthesia, small trauma, easily accepted, high safety, and easy to restore the patient’s hematopoietic system.OBJECTIVE:To observe the function and safety of autologous peripheral blood mononuclear cells in the treatment of patients with decompensated cirrhosis. METHODS:Four patients with decompensated liver cirrhosis were selected from November 2010 to July 2011 in the First Affiliated Hospital of Dalian Medical University, aged 31-67 (averagely 44 years). Among them, three cases had hepatitis B, and another one had autoimmune liver disease. Peripheral blood stem cells were collected after being mobilized by granulocyte colony stimulating factor. Then, autologous peripheral blood stem cells were transplanted via a hepatic artery catheter. RESULTS AND CONCLUSION:There were no adverse reactions such as fever, bleeding and nausea after peripheral blood stem cell collection and hepatic artery transplantation. Symptoms such as fatigue, poor appetite and abdominal distension gradually improved at 1, 3 and 6 months after transplantation. Liver function and liver fibrosis indexes were improved to some extent after transplantation.