pAKt在不明原因性不孕症妇女着床期子宫内膜的表达及意义

目的研究磷酸化AKt（pAKt）在不明原因性不孕症妇女着床期子宫内膜的表达变化及意义。方法收集18份原发性不孕症、不明原因性不孕症妇女黄体中期子宫内膜组织作为研究对象，并收集30份继发性不孕症、双侧输卵管阻塞患者黄体中期子宫内膜组织作为对照。用SP免疫组化法确定两组患者子宫内膜pAKt与白血病抑制因子（UV）的组织学定位及半定量表达。结果LIF在两组患者子宫内膜的表达均主要定位在腔上皮和腺上皮胞浆，pAKt在两组患者子宫内膜的表达主要位于间质细胞且呈胞浆强表达。两组患者子宫内膜LIF与pAKt蛋白表达半定量分析（sP免疫组化法）：不明原因性不孕症患者子宫内膜LIF与pAKt表达量较双侧输卵管阻塞患者明显降低（P〈0．05），两组患者中IVF／ICSI后未妊娠者子宫内膜LIF与pAKt表达量较妊娠者明显降低（P〈0．01）。结论子宫内膜容受性降低是不明原因性不孕症患者不孕原因之一，着床期子宫内膜pAKt表达降低可能是不孕症患者子宫内膜容受性降低的原因之一。

The expression of phosphorylated Akt and its significance in the endometrium of the women with unexplained infertility during the period of "implantation window"

Objective： To study on the expression of phosphorylated Akt and its significance in the endometrium of the women with unexplained infertility during the period of ＂implantation window＂ Methods： 18 samples of the endometrium in midterm of the luteum from the women with primary and unexplained infertility were collected as test group, and 30 samples of the endometrium in midterm of the luteum from the patients with secondary infertility and two fallopian tube obstructed were also collected as control group. SP immunohistochemistry assay was used to determine the tissue location of pAKt and LIF and their semi - quantitative expression of the endometrium in the two groups. Results： LIF expression were located in endometrial glandular epithelial cells and luminal epithelial cells of the two group, the expression of pAKt markedly located in stromal cells and cytoplasm showed strong expression in the patients＇endometrium of the two groups. Endometrial LIF and pAKt protein semi -quantitative expression in the two groups （SP immunohistochemistry assay） re-vealed as followed： compared with the patients with two fallopian tube obstructed, LIF and pAKt protein expression were both significantly decreased in the unexplained infertile women （ P 〈0. 05）, the expressions of LIF and pAKt protein in the pregnant patients after IVF/ICSI in the two groups were both significantly higher than those of the non -pregnant patients （ P 〈0.01 ）. Conclusion： Decreasing of endometrium receptivity is one of the infertile causes of women with unexplained infertility, decreased expression of pAKt in the endometrium during ＂implantation window＂ may be one of the causes of infertile women＇s decreased endometrium receptivity.