Relationship between Clinical Symptoms and the Microbiota in Advanced Caries |
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| Affiliation: | 1. Department of Endodontics, School of Stomatology, Tianjin Medical University, Tianjin, China;2. Department of Stomatology, Tianjin First Central Hospital, Tianjin, China;3. Department of Stomatology, Wuqing People Hospital, Tianjin, China;1. Department of Restorative, Preventive and Pediatric Dentistry, School of Dental Medicine, University of Bern, Bern, Switzerland;2. Department of Periodontology and Operative Dentistry, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany;3. Department of Prosthetic Dentistry, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany;4. Department of BioMedical Research, University of Bern, Bern, Switzerland;1. Department of Endodontics and Dental Research Group, Iguaçu University, Nova Iguaçu, Rio de Janeiro, Brazil;2. Private Practice, Cetraro, Italy;3. Postgraduate Program in Dentistry, University of Grande Rio, Duque de Caxias, Rio de Janeiro, Brazil;1. Department of Restorative, Preventive and Pediatric Dentistry, School of Dental Medicine, University of Bern, Bern, Switzerland;2. Department of Periodontology and Operative Dentistry, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany;3. Department for BioMedical Research, University of Bern, Bern, Switzerland;4. Reconstructive Dentistry and Gerodontology, School of Dental Medicine, University of Bern, Bern, Switzerland;6. Division of Preventive Dentistry, Periodontology, and Cariology, University of Zürich Center of Dental Medicine Zürich, Zurich, Switzerland;1. Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, Brazil;2. Department of Biological Sciences, Dental School of Bauru, University of São Paulo, Bauru, Brazil;1. Department of Endodontics, Texas A&M College of Dentistry, Dallas, Texas;2. Department of Biomedical Sciences, Texas A&M College of Dentistry, Dallas, Texas |
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| Abstract: | IntroductionThe composition and relative abundance of bacterial species change throughout the development of dental caries; however, how these changes relate to clinical symptoms remains elusive. In this study, we explored the relationship between clinical symptoms and specific microorganisms in advanced dentinal caries.MethodsA total of 111 permanent premolars and molars were used to simulate the progression from caries to pulpitis indirectly. Clinical symptoms were evaluated, and teeth were diagnosed according to the diagnostic criteria of the American Association of Endodontics. Samples were collected for 16S ribosomal DNA sequencing. Associations between the microbiota and clinical symptoms/diagnosis and the relationship between alpha diversity and clinical symptoms/diagnosis were evaluated independently by the linear discriminant analysis effect size and Spearman rank correlation analyses.ResultsThe 16S ribosomal DNA sequences were assigned to 13,852 operational taxonomic units. The linear discriminant analysis effect size and Spearman correlations unveiled negative associations between the relative abundance of Bacteroidia and Gammaproteobacteria and referred pain, Gammaproteobacteria and the electric pulp test response, and Actinomyces and Propionibacterium and diagnosis (r < 0.0, P < .05). Conversely, the relative abundance of Lactobacillus was positively correlated to referred pain, the cold test, the percussion response, and diagnosis (r > 0, P < .05). Lactobillus reuteri, a probiotic bacterium, was more abundant in teeth with referred pain and teeth diagnosed with symptomatic irreversible pulpitis. The Spearman correlation between alpha diversity and clinical symptoms/diagnosis was not significant (P > .05).ConclusionsClinical symptoms and diagnosis were significantly associated with specific microorganisms in the most advanced layers of dentinal caries. |
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| Keywords: | 16S ribosomal DNA dentinal caries microbiota pulpitis symptom |
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