| 比色法测定麦冬果实中总皂苷元含量 |
| |
| 引用本文： | 刘玉凤,王保国,温新民,崔晓秋.比色法测定麦冬果实中总皂苷元含量[J].济宁医学院学报,2009,32(5):310-311. |
| |
| 作者姓名： | 刘玉凤 王保国 温新民 崔晓秋 |
| |
| 作者单位： | 济宁医学院药学院,山东,日照,276826 |
| |
| 摘 要： | 目的建立麦冬果实中总皂苷元的含量测定方法。方法以香草醛-高氯酸为显色剂，薯蓣皂苷元为对照品，采用比色法测定。结果吸收度与对照品浓度在2．25μg／mL～7．5μg／mL范围内呈良好线性关系，线性回归方程为A=0．0492C＋0．044（r=0．999），平均加样回收率为98．8％，RSD=1．56％（n=6）。结论本方法准确、重复性好，可用于麦冬果实中总皂苷元的含量测定。
|
| 关 键 词： | 麦冬果实 薯蓣皂苷元 总皂苷元 比色法 含量测定 |
Determination of Total Sapogenins Content in the Fruits of Ophiopogon Japonicus by Colorimetric Method |
| |
| Institution: | LIU Yu-feng ,WANG Bao-guo ,WEN Xin-min ,et al ( Phamaceutlcal Science School, Jining Medical University, Rizhao 276826, China ) |
| |
| Abstract: | Objective To establish all method for determination of total sapogenins content in the fruits of Ophiopogon japonicus. Methods Colorimetric method analysis was employed. Vanillin-HClO4 worked as colour developing reagent, and diosgenin was used as the control sample. Results A good linear relationship was achieved over the concentration range of 2.25μg/mL-7.5μg/mL for diosgenin with an average recovery of 98.8%, RSD 1.56 % （n=6）. The equation of linear regression was A= 0. 0492C＋0. 044（r= 0. 999）Conclusion The method was accurate and reproducible, and it can be used to determine the total sapogenins content in the fruits of Ophiopogon japonicus. |
| |
| Keywords: | Fruit of Ophiopogon japonicus Diosgenin Total sapogenins Colorimetric method Content determination |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
