太阴病脾虚寒湿证大鼠模型的建立与机理探讨

目的:探求太阴病脾虚寒湿证客观性辨证指标。方法:实验大鼠连续14天每日经口灌服大黄与猪油造太阴病脾虚寒湿证模型。观察该模型大鼠的活动、体温等基本情况;取大鼠血用间苯三酚法测大鼠小肠对D-木糖(D-xylose)的吸收功能,能量代谢相关酶钠-钾-ATP酶(Na -K -ATPase)活性并观察胃、肝、脾、胰腺、小肠、结肠组织形态学改变。结果:模型大鼠活动减少,体温略低,小肠吸收D-木糖的功能减弱,Na -K -ATP酶的活性降低,胃黏膜萎缩,小肠肠上皮细胞轻度肿胀,肝细胞明显萎缩、肝细胞排列紊乱,胰腺细胞深染、内外分泌腺基本正常,结肠细胞核固缩、排列紊乱,脾脏体积显著缩小、脾小体数目减少。结论:D-木糖的吸收率,Na -K -ATP酶的活性以及胃、肝、小肠、脾、结肠的部分组织形态学改变可作为此证的客观诊断指标。

Building a White Rat Model to Investigate the Mechanism of the Cold-dampness Due to Spleen Insufficiency

Objective:Finding the indices for syndrome differentiation of Cold-dampness due to spleen insufficiency in Taiyin disease.Methods:Building up an animal model to recreate syndrome of cold-dampness due to spleen insufficiency by means of drenching Dahuang(radix et rhizoma rhei) and lard oil per os for 14 days.The manifestations of the rats are observed,including the basic conditions such as their daily activities and temperature,and absorption function of the small intestine to D-xylose,the activity of Na -K -ATP,and the tectological change of the stomach,small intestine,liver,pancreas,colonic,spleen,etc.by means of Phloroglucin method with rat's blood.Results:The rats in the model appear lazy with the temperature being low than usual and the absorptivity of the small intestine to D-xylose decreased.The enzyme activity of,Na -K -ATP reduces with atrophy of gastric mucosa.The epithelial cell of the small intestine is slightly swelling.The liver cells shrink and the pancreas cells engrain.The endo-exocrine glands are in normal level but the volume of the spleen reduces remarkably.Conclusion:The indices for diagnosis of this syndrome may include the absorptivity of D-xylose,the enzyme acitivity of Na -K -ATP,the morphological changes of the stomach,liver,the small intestine,spleen,etc.