脑室注射血管紧张素II对雄性大鼠下丘脑促性腺激素释放激素含量的影响

本实验观察了脑室注射血管紧张素Ｉ（ＡＩ）对成年雄性Ｗｉｓｔａｒ大鼠下丘脑促性腺激素释放激素（ＧｎＲＨ）含量的影响。摘取下丘脑组织，匀浆化，用放射免疫分析检测匀浆上清中ＧｎＲＨ的含量。结果表明，脑室注射ＡＩ（１２０ｎｇ／２０μｌ）后６０、９０及１２０分钟，下丘脑ＧｎＲＨ含量分别为２．８８±０．４７、２．６９±０．３８及２．５４±０．５５ｎｇ／１０ｍｇ湿重组织，均显著高于盐水对照组（Ｐ＜０．０５），提示ＡＩ可促进雄性大鼠下丘脑ＧＮＲＨ的生成。

Effect of intracerebroventricular injection of angiotensin II on hypothalamic gonadotrophin releasing hormone content in male rats

Objectives: To investigate the effect of intracerebroventricular injection of angiotensin II on hypothalamic gonadotrophin releasing hormone (GnRH) production in male rats. Design: Experimental animal study. Settings: Harbin Medical University. Methods: Male rats were anaesthetized with ether, then the saline (20 μl) and A II solution (at 30, 60 and 120 ng/20 μl) were injected intracerebroventricularly. The hypothalamic tissues were removed at 60, 90 and 120 min after intracerebroventricular injection of A II, followed by homogenization and centrifugation. The GnRH content in supernates of hypothalamic homogenates were measured by RIA. Results: The mean hypothalamic GnRH contents were 2.88±0.47, 2.69±0.38 and 2.54±0.55 ng/10mg wet tissue at 60, 90 and 120 min after intracerebroventricular injection of 120 ng A II, which were significantly higher than those in saline control groups (P<0.05). Conclusions: These results demonstrated that A II can stimulate GnRH production in the hypothalamus of male rat.