泻肺平喘灵的血清药物化学成分

目的:利用血清药物化学研究儿科复方制剂泻肺平喘灵的主要药效物质基础。方法:采用UPLC-LTQ-Qrbitrap技术,建立复方提取液和大鼠给药后血清的指纹图谱,并对比推断特征峰所归属的单味药及其成分。结果:检测并鉴定了泻肺平喘灵复方大鼠血中移行成分18个,其中10个成分被机体直接吸收入血;其余8个可能为原型成分经过机体代谢新产生的成分。结论:泻肺平喘灵的UPLC指纹图谱包含较多中药的血中移行成分的基本信息,可运用中药血清药物化学的研究方法进行复方药效物质基础的研究。

Study on Serum Pharmacochemistry of Xiefei Pingchuanling Extract

Objective: This article aimed to to study the main pharmacodynamic substances of pediatric compound preparation Xiefei Pingchuanling by serum pharmacochemistry. Method: UPLC-LTQ-Qrbitrap technology was used to create fingerprints of both compound extract and rat serum after treatment. Then the peaks were compared and predicted to belong to which single herb and their constituents were inferre. Result: 18 transitional ingredients in Xiefei Pingchuanling were detected and identified in rat serum, 10 of which could be directly absorbed into the bloodstream by the body;the 8 remaining prototype components may be new ingredients produced through metabolism. Conclusion: UPLC fingerprint provides much medicinal information in plasma of rats treated with Xiefei Pingchuanling, therefore, it is of great value to studies on the metabolism of traditional Chinese medicine (TCM).