|
|||||
|
|
| GIT1发夹结构干扰MLC的分布及其磷酸化 | |
| 引用本文： | 李〓翔,张〓宁,殷国勇,范卫民. GIT1发夹结构干扰MLC的分布及其磷酸化[J]. 南京医科大学学报(自然科学版), 2010, 0(4): 429-432 |
| 作者姓名： | 李〓翔 张〓宁 殷国勇 范卫民 |
| 作者单位： | 南京医科大学第一附属医院骨科; |
| 基金项目： | 国家自然科学基金资助(30670876);;江苏省自然科学基金资助(BK2006249) |
| 摘 要： | 目的:探索G蛋白偶联受体激酶结合蛋白1(G protein coupled receptor kinase interacting protein1,GIT1)的RNA发夹结构(hairpin)(GIT1-RNAh)对成骨细胞内肌球蛋白轻链(myosinlightchain,MLC)的分布及磷酸化的影响,并分析其机制。方法:取培养至第6代的鼠成骨细胞,随机分成两组,分别用包含GIT1-RNAh(实验组)和绿色荧光蛋白(green fluoresence protein,GFP)的发夹结构(GFP-RNAh)的腺病毒(对照组)感染12h,再将每组分成有、无血小板衍生生长因子(platelet-drived growth factor,PDGF)刺激的两组,免疫荧光染色方法检测成骨细胞内MLC的位置,Western blot检测MLC的磷酸化。结果:免疫组织化学观察:和对照组相比,实验组明显扰乱了成骨细胞MLC的分布;Western blot观察:和对照组相比,实验组明显抑制了MLC的磷酸化(P<0.05)。结论:GIT1的发卡结构过表达可干扰MLC的分布和其磷酸化。 |
| 关 键 词： | G蛋白偶联受体激酶结合蛋白1 MLC 血小板衍生生长因子 |
| 收稿时间： | 2009-11-19 |
GIT1 RNA hairpin interferes with MLC distributing and decreases MLC phosporylation in osteoblasts |
|
| LI Xiang,ZHANG Ning,YIN Guo-yong and FAN Wei-min. GIT1 RNA hairpin interferes with MLC distributing and decreases MLC phosporylation in osteoblasts[J]. Acta Universitatis Medicinalis Nanjing, 2010, 0(4): 429-432 | |
| Authors: | LI Xiang ZHANG Ning YIN Guo-yong FAN Wei-min |
| Affiliation: | Department of Orthopaedics/a>;the First Affiliated Hospital of NJMU/a>;Nanjing 210029/a>;China |
| Abstract: | Objective:To study the effect of G protein coupled receptor kinase interacting protein 1 (GIT1) RNA hairpin (GIT1-RNAh) on Myosin light chain (MLC) distribution and phosphorylation in osteoblasts. Methods:The sixth passage osteoblasts were divided into two groups infected with GIT1-RNAh (experimental group) and Green Fluoresence protein RNA hairpin (GFP-RNAh) (control group) adenovirus for 12 hours respectively. Each group was further classfied into two groups according to PDGF stimulation or not. MLC distr... |
| Keywords: | GIT1 MLC PDGF |
| 本文献已被 CNKI 等数据库收录！ | |
| 点击此处可从《南京医科大学学报(自然科学版)》浏览原始摘要信息 | |
| 点击此处可从《南京医科大学学报(自然科学版)》下载全文 | |