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| 小鼠核心结合因子1成熟肽编码区的cDNA克隆和序列测定 | |
| 引用本文： | 余擎,肖明振,吴补领,田宇,郭红延. 小鼠核心结合因子1成熟肽编码区的cDNA克隆和序列测定[J]. 牙体牙髓牙周病学杂志, 2002, 12(3): 121-123 |
| 作者姓名： | 余擎 肖明振 吴补领 田宇 郭红延 |
| 作者单位： | 第四军医大学口腔医学院,陕西,西安,710032 |
| 基金项目： | 全军“十五”计划基金重点课题 ( 0 12 0 89)，全军骨干青年教师资助项目 |
| 摘 要： | 目的：研究核心结合因子1（cbfal)在小鼠牙胚组织中的表达，扩增cbfal成熟肽编码区的特异性片段，构建重组质粒，为进一步的蛋白表达、抗体制备、基因转染等研究奠定基础。方法：提取新生小鼠的颅骨、牙胚、肝脏组织总RNA，反转录为cDNA后进行PCR反应，构建pGEM-cbfal重组质粒并测序。结果：从小鼠颅骨和牙胚组织中均获得了约1791bp的目的片段，牙胚来源的PCR产物的测序结果证实成功地克隆到了cbfal成熟肽编码区，酶切结果显示重组质粒构建成功。结论：新生小鼠的牙胚组织中有cbfal基因的表达。 |
| 关 键 词： | cDNA克隆 序列测定 小鼠 核心结合因子 聚合酶链式反应 蛋白表达 抗体制备 |
| 文章编号： | 1005-2593(2002)03-0121-03 |
| 修稿时间： | 2001-10-22 |
Cloning and sequencing of the coding region of mouse cbfa1 gene |
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| YU Qing,XIAO Ming-zhen,WU Bu-ling,et al. Cloning and sequencing of the coding region of mouse cbfa1 gene[J]. Chinese Journal of Conservative Dentistry, 2002, 12(3): 121-123 | |
| Authors: | YU Qing XIAO Ming-zhen WU Bu-ling et al |
| Abstract: | AIM: To observe the expression of cbfa1 in mouse tooth germs, to clone and sequence the cDNA of cbfa1 from mouse tooth germs. METHODS: Total RNA was isolated from newborn mouse tooth germs tissue and reverse-transcribed into cDNA. The desired DNA product was obtained by PCR with two cbfa1 specific primers. The segment was inserted into pGEM-5zf vector and the positive clone was sequenced by Takara Corporation. RESULTS: cDNA of cbfa1 was obtained from mouse tooth germs tissue. The sequence was consistent with that displayed on Pubmed. No mutation was found. CONCLUSION: This study provides the evidence of expression of cbfa1 in mouse tooth germs tissue. |
| Keywords: | mouse cbfa1 PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |