| HPLC、UPLC、CZE测定淫羊藿中黄酮类成分含量的比较研究 |
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| 引用本文： | 陈肖家,李绍平,王一涛,屠鹏飞. HPLC、UPLC、CZE测定淫羊藿中黄酮类成分含量的比较研究[J]. 中国药学杂志, 2008, 43(8): 624-628 |
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| 作者姓名： | 陈肖家 李绍平 王一涛 屠鹏飞 |
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| 作者单位： | 1.北京大学药学院天然药物学系 北京 100083;2.澳门大学中华医药研究院 澳门 |
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| 摘 要： | 目的比较淫羊藿中黄酮类成分的含量测定方法。方法分别用高效液相色谱(HPLC)、超高效液相色谱(UPLC)、毛细管区带电泳(CZE)对淫羊藿中15种黄酮类成分进行含量测定,并比较3种方法的分离情况、线性、灵敏度、精密度、重复性、加样回收率和样品测定结果。结果分析时间为UPLC
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| 关 键 词： | 淫羊藿 黄酮 高效液相色谱法 超高效液相色谱法 |
| 文章编号： | 1001-2494(2008)08-0624-05 |
| 收稿时间： | 2007-11-06; |
| 修稿时间： | 2007-11-06 |
Comparison of Quantitative Determination of Flavonoids in Herba Epimedii by HPLC,UPLC and CZE |
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| CHEN Xiao-jia,LI Shao-ping,WANG Yi-tao,TU Peng-fei. Comparison of Quantitative Determination of Flavonoids in Herba Epimedii by HPLC,UPLC and CZE[J]. Chinese Pharmaceutical Journal, 2008, 43(8): 624-628 |
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| Authors: | CHEN Xiao-jia LI Shao-ping WANG Yi-tao TU Peng-fei |
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| Affiliation: | 1.Department of Natural Medicines，School of Pharmaceutical Sciences，Peking University，Beijing 100083，China； 2.Institute of Chinese Medical Sciences，University of Macau，Macao，China |
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| Abstract: | OBJECTIVE To compare the methods for determination of flavonoids in Herba Epimedii.METHODS Fifteen flavonoids in Herba Epimedii were determined by HPLC,UPLC and CZE,respectively.And the separation,sensitivity,precision,repeatability,recovery and the contents of investigated compounds determined by the three methods were compared.RESULTS Analysis time of the three methods were UPLC
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| Keywords: | Herba Epimedii flavonoids high-performance liquid chromatography ultra-performance liquid chromatography capillary zone electrophoresis |
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