蛋白芯片法检测弓形虫的临床应用

目的评价蛋白芯片法诊断致畸病原体弓形虫的临床应用价值。方法①将蛋白芯片法与ELISA试剂对230份被检标本作平行检测;②用蛋白芯片法对卫生部室间质评的样本进行检测。结果蛋白芯片法与ELISA法各项指标检测结果均具有较好的一致性(P>0.05),弓形虫的阴性、阳性符合率均为100%;而对室间质评样本的检测结果则与卫生部所公布结果完全一致。结论蛋白芯片法检测弓形虫-IgM抗体,具有简便、快速,敏感性较高和特异性强等优点,是临床优生优育辅助诊断的有效方法,值得推广使用。

The Clinical Application of Protein Chip for Toxoplasma Gondii Detection

Objective To evaluate the clinical value of protein chip in the diagnosis of toxoplasma gondii that is a kind of teratogenic pathogen.Methods ①230 samples were detected by protein chip and ELISA reagent at the same time.②The external quality assessment samples from the Ministry of Health were tested by protein chip.Results The detection results of various indexes had good consistency by ELISA and protein chip(P>0.05).The coincidence rates of toxoplasma gondii negative and toxoplasma gondii positive were 100%.The detection results of external quality control samples were consistent with the results from the Ministry of Health.Conclusion Protein chip for toxoplasma-IgM antibodies detection is simple,rapid,has the advantages of high sensitivity and specificity,and it is an effective assist diagnosis method for prenatal and postnatal care,which is worth being widely used.