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Effects of Glucose Dehydrogenase in Converting Urea and Ammonia into Amino Acid Using Artificial Cells
Authors:Thomas M. S. Chang  and Celeste Malouf
Affiliation:Artificial Organs Research Unit, MeIntyre Medical Sciences Building, McGill Uniuersity, 3655 Drummond Street, Montreal, Quebec, Canada
Abstract:
A microencapsulated multienzyme system containing urease, glutamate dehydrogenase and glucose dehydrogenase has been used to convert urea and ammonia into an amino acid. The effect of two different glucose dehydrogenases was studied in detail. High-specific-activity glucose dehydrogenase requires minimal cofactor and glucose and can greatly facilitate the further development of this approach for possible clinical applications.
Keywords:urea    ammonia    amino acid from urea    amino acid from ammonia    glutamate    artificial cells    immobilized multienzyme    microcapsules
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