Effects of Glucose Dehydrogenase in Converting Urea and Ammonia into Amino Acid Using Artificial Cells |
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Authors: | Thomas M. S. Chang and Celeste Malouf |
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Affiliation: | Artificial Organs Research Unit, MeIntyre Medical Sciences Building, McGill Uniuersity, 3655 Drummond Street, Montreal, Quebec, Canada |
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Abstract: | A microencapsulated multienzyme system containing urease, glutamate dehydrogenase and glucose dehydrogenase has been used to convert urea and ammonia into an amino acid. The effect of two different glucose dehydrogenases was studied in detail. High-specific-activity glucose dehydrogenase requires minimal cofactor and glucose and can greatly facilitate the further development of this approach for possible clinical applications. |
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Keywords: | urea ammonia amino acid from urea amino acid from ammonia glutamate artificial cells immobilized multienzyme microcapsules |
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