斑蝥酸钠和肿节风对腹水型肝癌H22细胞及荷瘤小鼠肝脏能量代谢与环核苷酸代谢的影响

实验结果表明:斑蝥酸钠和肿节风能改善肿瘤细胞和荷瘤小鼠的能量代谢,提高过氧化氢酶活力,即降低荷瘤小鼠的癌毒激素水平,故改善能量代谢可能是它们控制和缓解癌变的途径之一;H22癌细胞的 cAMP 与 cGMP 的比值明显低于正常鼠肝,经斑蝥酸钠治疗后的荷瘤小鼠癌细胞的 cAMP 和 cAMP/cGMP 均高于未经治疗的荷瘤小鼠,同时测得癌细胞 cAMP-磷酸二酯酶(低 Km)明显下降。因此,斑蝥酸钠的抗癌作用可能与降低cAMP-磷酸二酯酶活性从而提高癌细胞的 cAMP 有关。

Effect of Disodium Cantharidate and Injectio Herbae Sar Candrae on Energy and Cyclic Nucleotide Metabolism in Hepatoma 22 Cells and Liver Tissues of Tumor-Bearing Mice

Effect of Disodium Cantharidate(DSC)and injectio herbae Sarcandrae on oxygen consumption,respiratory control ratio,oxidative phosphorylation efficiency,succinate dehydrogenase activity,catalase activity,cAMP and cGMP levels and phosphodiesterase aetivities in Hepatoma 22 cells and liver tissues of tumor-bearing mice were studied in vivo and in vitro. The present data suggest that these two drugs are respiration inhibitors and can improve the energy metabolism of tumor cells and tumor-bearing mice.They also increase the catalase activity,showing a decrease in toxohormone level of these mice.The improvement of energy metabolism may be one of their ways to control or moderate carcinogenesis. The cAMP/cGMP ratio was found much lower in Hepatoma cells than in livers of normal mice.It was also found that DSC not only elevated the intracellular cAMP level and cAMP/cGMP ratio,but also inhibited cAMP phosphodiesterase activity(low Km)in Hepatoma cells.These results suggest that the antitumor mechanism of DSC may be partly due to modulating cAMP level by inhibiting the activity of cAMP phosphodiesterase.