Immunohistochemistry of collagen types II and X, and enzyme-histochemistry of alkaline phosphatase in the developing condylar cartilage of the fetal mouse mandible |
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| Authors: | SHUNICHI SHIBATA KENJI FUKADA SHOICHI SUZUKI YASUO YAMASHITA |
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| Affiliation: | 1st Department of Oral Anatomy, School of Dentistry, Tokyo Medical and Dental University, Tokyo, Japan;2nd Department of Orthodontics, School of Dentistry, Tokyo Medical and Dental University, Tokyo, Japan |
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| Abstract: | We investigated the immunohistochemical localisation of types II and X collagen as well as the cytochemical localisation of alkaline phosphatase in the developing condylar cartilage of the fetal mouse mandible on d 14–16 of pregnancy. On d 14 of pregnancy, although no immunostaining for types II and X collagen was observed, alkaline phosphatase activity was detected in all cells in the anlage of the future condylar process. On d 15 of pregnancy, immunostaining for both collagen types was simultaneously detected in the primarily formed condylar cartilage. Alkaline phosphatase activity was also detected in chondrocytes at this stage. By d 16 of pregnancy, the hypertrophic cell zone rapidly increased in size. These findings strongly support a periosteal origin for the condylar cartilage of the fetal mouse mandible, and show that progenitor cells for condylar cartilage rapidly or directly differentiate into hypertrophic chondrocytes. |
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| Keywords: | Bone periosteum chondrocytes |
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