燃煤型氟骨症早期大鼠血清PTH、CT的变化

目的研究燃煤型氟骨症早期血清甲状旁腺激素(PTH)、降钙素(CT)的变化。方法将SD大鼠随机分为6组:对照、低氟、中氟加营养、中氟、高氟加营养、高氟组,每组34只,雌雄各半。各染毒组喂饲含不同比例的燃煤型病区煤烘玉米的饲料复制氟中毒动物模型。分2批以股动脉放血法处死动物,检查氟斑牙,测尿、骨、肾氟,骨密度(BMD),骨Ca、尿Ca,血清PTH、CT。结果(1)建成氟中毒动物模型:各染毒组均出现氟中毒,对照组正常。中毒严重程度随氟剂量增加而加重;氟剂量相同时,营养好,中毒程度轻。(2)燃煤型氟中毒对骨代谢相关激素的影响:高氟组血清PTH后期高于早期,差异有统计学意义(P<0.01)。说明染氟大鼠成骨活动增强,该指标变化较晚。血清CT各组早期无变化,后期低氟组和中营组升高,差异有统计学意义(P<0.05或0.01);低氟组高于高氟组,差异有统计学意义(P<0.05)。结论(1)骨转换活跃可能是燃煤型氟中毒骨病变发生发展的一个重要环节并且早期以成骨活跃为主;(2)尚不能认为血清PTH、CT是反映燃煤型氟骨症病情的早期指标;(3)降低摄氟量及改善营养状况,可缓解氟中毒病情。

Change of parathyroid hormone and calcitonin in serum at early phase of skeletal fluorosis caused by fluoride in coal in rats

Objective To study the change of parathyroid hormone(PTH) and calcitonin(CT)in serum at the early phase of skeletal fluorosis caused by fluoride in coal.Methods SD rats were taken as the experimental objects,being random assorted into 6 groups(the number either female or male was same in each group):control group,lowdose fluoride group,middle-dose fluoride and added nutrition group,middle-dose fluoride group,high-dose fluoride and added nutrition group,high-dose fluoride group.All rats were killed by means of arteria femoralis phlebotomy,then were examined dental fluorosis,fluorine in urine,bone,kidney,bone mineral density(BMD),calcium in bone and urine,PTH and CT in serum.Results(1)The estabolished animal model of fluorosis was successful.There were fluorosis in every test group and it's all right for rats in the control group.The higher the fluoride dose,the more serious fluorosis syndrome of the rats.On the condition of same fluoride dose,the fluorosis syndrome was mild if nutrition was good.(2)The effect of coal-burning-fluorosis on hormones correlated with skeletal metabolism a.PTH in serum PTH of the high-dose group at the latter phase was higher than that at the earlier phase(P<0.01);b.CT in serum At the earlier phase,CT had no manifest change;at the later phase,CT of the low-dose and the middle-dose and nutrition groups were higher than that of the control group(P<0.05 or 0.01),CT of the low-dose group was higher than that of the high-dose group(P<0.05).Conclusion(1)The active of skeletal transduction probably is an important process in the genesis and development of fluorosis,at the earlier phase,skeletal formation active is more pantent.(2)Neither PTH nor CT could be taken as the earlier indexes of skeletal fluorosis caused by fluoride in coal so far,(3)Reducing fluoride intake and improving nutrition can be used to release sydromes of fluorosis.