| 金菊双花总黄酮提取与纯化工艺研究 |
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| 引用本文： | 杨俊,张晓静,田丽娟,王兴旺. 金菊双花总黄酮提取与纯化工艺研究[J]. 中药材, 2005, 28(8): 703-705 |
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| 作者姓名： | 杨俊 张晓静 田丽娟 王兴旺 |
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| 作者单位： | 金陵药业股份有限公司,南京210009 |
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| 摘 要： | 目的:筛选金菊双花总黄酮的最佳提取与纯化工艺.方法:采用多种提取工艺比较、L9(34)正交实验及D101大孔吸附树脂纯化技术.结果:其最佳提取工艺为8倍量55%乙醇、100℃水浴、每次1 h回流提取2次,再结合D101大孔吸附树脂纯化,水、30%乙醇、70%乙醇梯度洗脱,收集70%乙醇洗脱部分浓缩干燥并测定黄酮含量为62.7%.结论:此工艺简便可行,符合工业化生产要求.
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| 关 键 词： | 金银花 菊花 总黄酮 提取 纯化 |
| 收稿时间： | 2005-02-22 |
| 修稿时间： | 2005-02-22 |
Study on the Extraction and Purification for Total Flavonoids in Lonicera japonica Thunb. And Chrysanthemum morifolium Tzvel |
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| Yang Jun,Zhang Xiaojing,Tian Lijuan,WANG Xingwang. Study on the Extraction and Purification for Total Flavonoids in Lonicera japonica Thunb. And Chrysanthemum morifolium Tzvel[J]. Journal of Chinese medicinal materials, 2005, 28(8): 703-705 |
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| Authors: | Yang Jun Zhang Xiaojing Tian Lijuan WANG Xingwang |
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| Affiliation: | Jinling Pharmaceutical Co. Ltd, Nanjing, Jiangsu. |
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| Abstract: | OBJECTIVE: To optimize the extraction and purification conditions for total flavonoids in Lonicera japonica Thunb. and Chrysanthemum morifolium Tzvel. METHODS: Comparision of several extraction processes, the L9 (3(4)) orthogonal design and macroreticular absorption resin D101 purification technique were performed. RESULTS: The optimum extraction conditions were 8 volume of 55% ethanol, 1 h refluence at 100 degrees C , water bath for 2 times. The extracted fluid was absorbed with the resin D101. The elutions were water, 30% enthanol, 70% enthanol. The obtained elution of 70% enthanol was evaporated and the content of total flavonoids was 62. 7%. CONCLUSION: The method is simple, approved and fit for industrial production. |
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| Keywords: | Lonicera japonica Thunb. Chrysanthemum morifolium Tzvel. Total flavonoids Extraction Purification |
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