| 猪牙皂正丁醇提取物含药血清抑制人肝癌细胞 SMMC-7721 的实验研究 |
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| 引用本文： | 汪红卫,张赤志,许汉林,陈成,蔡岳,王晓东. 猪牙皂正丁醇提取物含药血清抑制人肝癌细胞 SMMC-7721 的实验研究[J]. 中西医结合肝病杂志, 2013, 0(5): 279-281 |
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| 作者姓名： | 汪红卫 张赤志 许汉林 陈成 蔡岳 王晓东 |
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| 作者单位： | [1]湖北中医药大学2010级博士生部,湖北武汉430061 [2]湖北省中医院 ,湖北武汉430061 [3]湖北中医药大学药学院 ,湖北武汉430061 [4]湖北中医药大学2012级博士生部,湖北武汉430061 |
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| 基金项目： | 中国中医科学院新药创制联合课题（2011XYCZ-13）;致谢感谢湖北中医药大学基础医学院预防医学教研室杨海军在统计学处理上的支持. |
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| 摘 要： | 目的：研究猪牙皂提取物正丁醇部位对人肝癌细胞SMMC-7721的抑制效应。方法：40只Wistar雄性大鼠分为4组，其中1组给予生理盐水，其余3组分别给予高、中、低剂量猪牙皂正丁醇提取物（0．6g·kg^-1·d^-1、0．3g·kg^-1·d^-1、0．15g·kg^-1·d^-1）灌喂3天，采集其含药血清作用于人肝癌SMMC-7721细胞，MTr法检测细胞抑制率。结果：猪牙皂正丁醇提取物中、低剂量明显抑制人肝癌SMMC-7721细胞，高剂量无明显效果。结论：猪牙皂正丁醇提取物能够抑制人肝癌SMMC-7721细胞。
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| 关 键 词： | 猪牙皂 皂荚 正丁醇 提取物 含药血清 药效基团 SMMC-7721细胞 抑制率 |
Inhibitory effects of medicated serum of gleditsia sinensis Lam n-butanol extract on human hepatoma cell SMMC-7721 |
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| Affiliation: | WANG HONG-WEI , ZHANG CHI-ZHI , XU HAN-LIN , et al. (12010 Doctoral, Hubei University of Chinese Medicine ( Wuhan Hubei, 430061 ) China 2. Hubei Provincial Hospital of TCM) |
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| Abstract: | Objective： To study the inhibitory effect of butanol extract of gleditsia sinensis Lam. on human hepatoma cell line SMMC-7721. Methods： Forty Wistar male rats were divided into four groups, one group was given normal saline gastrogavage as a control group and other three groups were given respectively by high （0. 6g·kg^-1·d^-1 ）, middle （0. 3g·kg^-1·d^-1 ）, and low （0. 15g·kg^-1·d^-1 ） dosage of butanol extract of gleditsia for three days, then collecting medicated serum to affect on human hepatoma cell line SMMC-7721. Inhibition ratio was calculated by MTF essay. Results： There were significantly on middle and low doses of butanol extract of gleditsia inhibited human hepatoma cell SMMC-7721, but no significant in high dosage group. Conclusion： Butanol extract of gleditsia can suppress human hepatoma cell line SMMC-7721. |
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| Keywords: | gleditsia sinensis Lam butanol extract medicated serum pharmacophore SMMC-7721 cells inhibition rate |
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