A highly sensitive enzyme-linked immunosorbent assay for idiotype-bearing antibodies |
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| Authors: | G Di Felice C Pini G Doria L Adorini |
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| Affiliation: | 1. Laboratory of Immunology, Istituto Superiore di Sanità, Rome, Italy;2. ENEA-Euratom Immunogenetics Group, Laboratory of Pathology, Centro Ricerche Energetiche Casaccia, Rome, Italy |
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| Abstract: | A sensitive and specific immunoenzyme assay (ELISA) for quantitation of total and cross-reactive idiotype-bearing (CRI) anti-ABA antibodies is described. Total anti-ABA antibodies are directly assessed in ABA-BGG coated polyvinyl wells with enzyme-labelled rabbit anti-mouse immunoglobulins. By interpolation on a standard curve absorbance values give the concentration of anti-ABA antibodies with a sensitivity of 30 ng/ml. CRI+ antibodies are quantitated by inhibition of enzyme-labelled monoclonal CRI+ antibody binding to solid-phase coated rabbit anti-CRI immunoglobulins. The concentration of CRI+ antibodies, evaluated by interpolation on a standard inhibition curve, can be measured at the level of 10 ng/ml. This highly sensitive, rapid, specific and reproducible assay is easily used, with minor modifications, to detect specific antibodies in any idiotype system. |
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| Keywords: | antibody idiotypes ELISA cross-reactive idiotypes azobenzene arsonate |
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